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Glycation and Oxidative Stress Increase Autoantibodies in the Elderly
Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance in the elderly. This study aims to understand links among aging, gluco-oxidative stress, and autoantibodies in asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemic...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466087/ https://www.ncbi.nlm.nih.gov/pubmed/32806692 http://dx.doi.org/10.3390/molecules25163675 |
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author | Khan, Mohd W.A. Al Otaibi, Ahmed Sherwani, Subuhi Khan, Wahid A. Alshammari, Eida M. Al-Zahrani, Salma A. Saleem, Mohd Khan, Shahper N. Alouffi, Sultan |
author_facet | Khan, Mohd W.A. Al Otaibi, Ahmed Sherwani, Subuhi Khan, Wahid A. Alshammari, Eida M. Al-Zahrani, Salma A. Saleem, Mohd Khan, Shahper N. Alouffi, Sultan |
author_sort | Khan, Mohd W.A. |
collection | PubMed |
description | Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance in the elderly. This study aims to understand links among aging, gluco-oxidative stress, and autoantibodies in asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemical changes. Significant inhibition of advanced glycation end products (AGEs) formation was achieved using garlic extract (53.75%) and epigallocatechin-3-gallate from green tea (72.5%). Increased amounts of serum carbonyl content (2.42 ± 0.5) and pentosidine (0.0321 ± 0.0029) were detected in IV-S (S represent smokers) vs. IV group individuals. Direct binding ELISA results exhibited significantly high autoantibodies against Gly-HSA in group IV-S (0.55 ± 0.054; p < 0.001) and III-S (0.40 ± 0.044; p < 0.01) individuals as compared to the age matched subjects who were non-smokers (group IV and III). Moreover, high average percent inhibition (51.3 ± 4.1%) was obtained against Gly-HSA in IV-S group individuals. Apparent association constant was found to be high for serum immunoglobulin-G (IgG) from group IV-S (1.18 × 10(−6) M) vs. serum IgG from IV group (3.32 × 10(−7) M). Aging induced gluco-oxidative stress and AGEs formation may generate neo-epitopes on blood-proteins, contributing to production of autoantibodies in the elderly, especially smokers. Use of anti-glycation natural products may reduce age-related pathophysiological changes. |
format | Online Article Text |
id | pubmed-7466087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74660872020-09-14 Glycation and Oxidative Stress Increase Autoantibodies in the Elderly Khan, Mohd W.A. Al Otaibi, Ahmed Sherwani, Subuhi Khan, Wahid A. Alshammari, Eida M. Al-Zahrani, Salma A. Saleem, Mohd Khan, Shahper N. Alouffi, Sultan Molecules Article Aging causes gradual changes in free radicals, antioxidants, and immune-imbalance in the elderly. This study aims to understand links among aging, gluco-oxidative stress, and autoantibodies in asymptomatic individuals. In vitro glycation of human serum albumin (Gly-HSA) induces appreciable biochemical changes. Significant inhibition of advanced glycation end products (AGEs) formation was achieved using garlic extract (53.75%) and epigallocatechin-3-gallate from green tea (72.5%). Increased amounts of serum carbonyl content (2.42 ± 0.5) and pentosidine (0.0321 ± 0.0029) were detected in IV-S (S represent smokers) vs. IV group individuals. Direct binding ELISA results exhibited significantly high autoantibodies against Gly-HSA in group IV-S (0.55 ± 0.054; p < 0.001) and III-S (0.40 ± 0.044; p < 0.01) individuals as compared to the age matched subjects who were non-smokers (group IV and III). Moreover, high average percent inhibition (51.3 ± 4.1%) was obtained against Gly-HSA in IV-S group individuals. Apparent association constant was found to be high for serum immunoglobulin-G (IgG) from group IV-S (1.18 × 10(−6) M) vs. serum IgG from IV group (3.32 × 10(−7) M). Aging induced gluco-oxidative stress and AGEs formation may generate neo-epitopes on blood-proteins, contributing to production of autoantibodies in the elderly, especially smokers. Use of anti-glycation natural products may reduce age-related pathophysiological changes. MDPI 2020-08-12 /pmc/articles/PMC7466087/ /pubmed/32806692 http://dx.doi.org/10.3390/molecules25163675 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Khan, Mohd W.A. Al Otaibi, Ahmed Sherwani, Subuhi Khan, Wahid A. Alshammari, Eida M. Al-Zahrani, Salma A. Saleem, Mohd Khan, Shahper N. Alouffi, Sultan Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title | Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title_full | Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title_fullStr | Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title_full_unstemmed | Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title_short | Glycation and Oxidative Stress Increase Autoantibodies in the Elderly |
title_sort | glycation and oxidative stress increase autoantibodies in the elderly |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466087/ https://www.ncbi.nlm.nih.gov/pubmed/32806692 http://dx.doi.org/10.3390/molecules25163675 |
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