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EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466360/ https://www.ncbi.nlm.nih.gov/pubmed/32945400 http://dx.doi.org/10.3892/mmr.2020.11384 |
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author | Wei, Qian Wei, Lijuan Zhang, Jingya Li, Ze Feng, Honglei Ren, Li |
author_facet | Wei, Qian Wei, Lijuan Zhang, Jingya Li, Ze Feng, Honglei Ren, Li |
author_sort | Wei, Qian |
collection | PubMed |
description | Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstrated that exosomes derived from the highly metastatic Panc-1 PC cell line were internalized by a low metastatic cell line, resulting in increased migration of the latter. Proteomics analysis further revealed that the receptor tyrosine kinase Eph receptor A2 (EphA2) was overexpressed in the Panc-1 exosomes, and these Exo_EphA2 had the ability to transfer metastatic potential to recipient cells. Consistent with this, circulating Exo_EphA2 levels were higher in patients with PC compared with healthy controls. Taken together, these results indicated that Exo_EphA2 acts an oncogene in PC and is a potential tumor maker for PC diagnosis. |
format | Online Article Text |
id | pubmed-7466360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-74663602020-09-03 EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer Wei, Qian Wei, Lijuan Zhang, Jingya Li, Ze Feng, Honglei Ren, Li Mol Med Rep Articles Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstrated that exosomes derived from the highly metastatic Panc-1 PC cell line were internalized by a low metastatic cell line, resulting in increased migration of the latter. Proteomics analysis further revealed that the receptor tyrosine kinase Eph receptor A2 (EphA2) was overexpressed in the Panc-1 exosomes, and these Exo_EphA2 had the ability to transfer metastatic potential to recipient cells. Consistent with this, circulating Exo_EphA2 levels were higher in patients with PC compared with healthy controls. Taken together, these results indicated that Exo_EphA2 acts an oncogene in PC and is a potential tumor maker for PC diagnosis. D.A. Spandidos 2020-10 2020-07-28 /pmc/articles/PMC7466360/ /pubmed/32945400 http://dx.doi.org/10.3892/mmr.2020.11384 Text en Copyright: © Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Wei, Qian Wei, Lijuan Zhang, Jingya Li, Ze Feng, Honglei Ren, Li EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title | EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title_full | EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title_fullStr | EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title_full_unstemmed | EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title_short | EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
title_sort | epha2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466360/ https://www.ncbi.nlm.nih.gov/pubmed/32945400 http://dx.doi.org/10.3892/mmr.2020.11384 |
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