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EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer

Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstr...

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Autores principales: Wei, Qian, Wei, Lijuan, Zhang, Jingya, Li, Ze, Feng, Honglei, Ren, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466360/
https://www.ncbi.nlm.nih.gov/pubmed/32945400
http://dx.doi.org/10.3892/mmr.2020.11384
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author Wei, Qian
Wei, Lijuan
Zhang, Jingya
Li, Ze
Feng, Honglei
Ren, Li
author_facet Wei, Qian
Wei, Lijuan
Zhang, Jingya
Li, Ze
Feng, Honglei
Ren, Li
author_sort Wei, Qian
collection PubMed
description Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstrated that exosomes derived from the highly metastatic Panc-1 PC cell line were internalized by a low metastatic cell line, resulting in increased migration of the latter. Proteomics analysis further revealed that the receptor tyrosine kinase Eph receptor A2 (EphA2) was overexpressed in the Panc-1 exosomes, and these Exo_EphA2 had the ability to transfer metastatic potential to recipient cells. Consistent with this, circulating Exo_EphA2 levels were higher in patients with PC compared with healthy controls. Taken together, these results indicated that Exo_EphA2 acts an oncogene in PC and is a potential tumor maker for PC diagnosis.
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spelling pubmed-74663602020-09-03 EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer Wei, Qian Wei, Lijuan Zhang, Jingya Li, Ze Feng, Honglei Ren, Li Mol Med Rep Articles Pancreatic cancer (PC) is the fourth most common cause of cancer-related mortality worldwide and is characterized by high invasiveness and early metastasis. To identify novel diagnostic markers, the present study aimed to understand the mechanism underlying PC progression. The present study demonstrated that exosomes derived from the highly metastatic Panc-1 PC cell line were internalized by a low metastatic cell line, resulting in increased migration of the latter. Proteomics analysis further revealed that the receptor tyrosine kinase Eph receptor A2 (EphA2) was overexpressed in the Panc-1 exosomes, and these Exo_EphA2 had the ability to transfer metastatic potential to recipient cells. Consistent with this, circulating Exo_EphA2 levels were higher in patients with PC compared with healthy controls. Taken together, these results indicated that Exo_EphA2 acts an oncogene in PC and is a potential tumor maker for PC diagnosis. D.A. Spandidos 2020-10 2020-07-28 /pmc/articles/PMC7466360/ /pubmed/32945400 http://dx.doi.org/10.3892/mmr.2020.11384 Text en Copyright: © Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wei, Qian
Wei, Lijuan
Zhang, Jingya
Li, Ze
Feng, Honglei
Ren, Li
EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title_full EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title_fullStr EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title_full_unstemmed EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title_short EphA2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
title_sort epha2-enriched exosomes promote cell migration and are a potential diagnostic serum marker in pancreatic cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466360/
https://www.ncbi.nlm.nih.gov/pubmed/32945400
http://dx.doi.org/10.3892/mmr.2020.11384
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