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Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread

Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of conc...

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Autores principales: K. Anpalagan, Karthiga, V. Karakkat, Jimsheena, Truskewycz, Adam, Saedi, Ahmed Al, Joseph, Paul, Apostolopoulos, Vasso, Nurgali, Kulmira, Cole, Ivan, Cai, Zibo, T. H. Lai, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466409/
https://www.ncbi.nlm.nih.gov/pubmed/32796659
http://dx.doi.org/10.3390/nano10081575
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author K. Anpalagan, Karthiga
V. Karakkat, Jimsheena
Truskewycz, Adam
Saedi, Ahmed Al
Joseph, Paul
Apostolopoulos, Vasso
Nurgali, Kulmira
Cole, Ivan
Cai, Zibo
T. H. Lai, Daniel
author_facet K. Anpalagan, Karthiga
V. Karakkat, Jimsheena
Truskewycz, Adam
Saedi, Ahmed Al
Joseph, Paul
Apostolopoulos, Vasso
Nurgali, Kulmira
Cole, Ivan
Cai, Zibo
T. H. Lai, Daniel
author_sort K. Anpalagan, Karthiga
collection PubMed
description Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before.
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spelling pubmed-74664092020-09-14 Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread K. Anpalagan, Karthiga V. Karakkat, Jimsheena Truskewycz, Adam Saedi, Ahmed Al Joseph, Paul Apostolopoulos, Vasso Nurgali, Kulmira Cole, Ivan Cai, Zibo T. H. Lai, Daniel Nanomaterials (Basel) Article Biocompatible carbon quantum dots (CQDs) have recently attracted increased interest in biomedical imaging owing to their advantageous photoluminescence properties. Numerous precursors of fluorescent CQDs and various fabrication procedures are also reported in the literature. However; the use of concentrated mineral acids and other corrosive chemicals during the fabrication process curtails their biocompatibility and severely limits the utilization of the products in cell bio-imaging. In this study; a facile; fast; and cost-effective synthetic route is employed to fabricate CQDs from a natural organic resource; namely bread; where the use of any toxic chemicals is eliminated. Thus; the novel chemical-free technique facilitated the production of luminescent CQDs that were endowed with low cytotoxicity and; therefore; suitable candidates for bioimaging sensors. The above mentioned amorphous CQDs also exhibited fluorescence over 360–420 nm excitation wavelengths; and with a broad emission range of 360–600 nm. We have also shown that the CQDs were well internalized by muscle myoblasts (C2C12) and differentiated myotubes; the cell lines which have not been reported before. MDPI 2020-08-11 /pmc/articles/PMC7466409/ /pubmed/32796659 http://dx.doi.org/10.3390/nano10081575 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
K. Anpalagan, Karthiga
V. Karakkat, Jimsheena
Truskewycz, Adam
Saedi, Ahmed Al
Joseph, Paul
Apostolopoulos, Vasso
Nurgali, Kulmira
Cole, Ivan
Cai, Zibo
T. H. Lai, Daniel
Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title_full Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title_fullStr Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title_full_unstemmed Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title_short Bioimaging of C2C12 Muscle Myoblasts Using Fluorescent Carbon Quantum Dots Synthesized from Bread
title_sort bioimaging of c2c12 muscle myoblasts using fluorescent carbon quantum dots synthesized from bread
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466409/
https://www.ncbi.nlm.nih.gov/pubmed/32796659
http://dx.doi.org/10.3390/nano10081575
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