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Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications

Full-thickness skin wounds are a significant clinical burden in the United States. Skin bioprinting is a relatively new technology that is under investigation as a new treatment for full-thickness injuries, and development of hydrogels with strong physical and biological characteristics are required...

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Autores principales: Jorgensen, Adam M, Chou, Zishuai, Gillispie, Gregory, Lee, Sang Jin, Yoo, James J, Soker, Shay, Atala, Anthony
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466410/
https://www.ncbi.nlm.nih.gov/pubmed/32751101
http://dx.doi.org/10.3390/nano10081484
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author Jorgensen, Adam M
Chou, Zishuai
Gillispie, Gregory
Lee, Sang Jin
Yoo, James J
Soker, Shay
Atala, Anthony
author_facet Jorgensen, Adam M
Chou, Zishuai
Gillispie, Gregory
Lee, Sang Jin
Yoo, James J
Soker, Shay
Atala, Anthony
author_sort Jorgensen, Adam M
collection PubMed
description Full-thickness skin wounds are a significant clinical burden in the United States. Skin bioprinting is a relatively new technology that is under investigation as a new treatment for full-thickness injuries, and development of hydrogels with strong physical and biological characteristics are required to improve both structural integrity of the printed constructs while allowing for a more normal extracellular matrix milieu. This project aims to evaluate the physical and biological characteristics of fibrinogen hydrogel supplemented with decellularized human skin-derived extracellular matrix (dsECM). The hybrid hydrogel improves the cell viability and structural strength of bioprinted skin constructs. Scanning electron microscopy demonstrates that the hybrid hydrogel is composed of both swelling bundles interlocked in a fibrin network, similar to healthy human skin. This hybrid hydrogel has improved rheological properties and shear thinning properties. Extrusion-based printing of the fibrinogen hydrogel + dsECM demonstrates significant improvement in crosshatch pore size. These findings suggest that incorporating the properties of dsECM and fibrinogen hydrogels will improve in vivo integration of the bioprinted skin constructs and support of healthy skin wound regeneration.
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spelling pubmed-74664102020-09-14 Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications Jorgensen, Adam M Chou, Zishuai Gillispie, Gregory Lee, Sang Jin Yoo, James J Soker, Shay Atala, Anthony Nanomaterials (Basel) Article Full-thickness skin wounds are a significant clinical burden in the United States. Skin bioprinting is a relatively new technology that is under investigation as a new treatment for full-thickness injuries, and development of hydrogels with strong physical and biological characteristics are required to improve both structural integrity of the printed constructs while allowing for a more normal extracellular matrix milieu. This project aims to evaluate the physical and biological characteristics of fibrinogen hydrogel supplemented with decellularized human skin-derived extracellular matrix (dsECM). The hybrid hydrogel improves the cell viability and structural strength of bioprinted skin constructs. Scanning electron microscopy demonstrates that the hybrid hydrogel is composed of both swelling bundles interlocked in a fibrin network, similar to healthy human skin. This hybrid hydrogel has improved rheological properties and shear thinning properties. Extrusion-based printing of the fibrinogen hydrogel + dsECM demonstrates significant improvement in crosshatch pore size. These findings suggest that incorporating the properties of dsECM and fibrinogen hydrogels will improve in vivo integration of the bioprinted skin constructs and support of healthy skin wound regeneration. MDPI 2020-07-29 /pmc/articles/PMC7466410/ /pubmed/32751101 http://dx.doi.org/10.3390/nano10081484 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jorgensen, Adam M
Chou, Zishuai
Gillispie, Gregory
Lee, Sang Jin
Yoo, James J
Soker, Shay
Atala, Anthony
Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title_full Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title_fullStr Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title_full_unstemmed Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title_short Decellularized Skin Extracellular Matrix (dsECM) Improves the Physical and Biological Properties of Fibrinogen Hydrogel for Skin Bioprinting Applications
title_sort decellularized skin extracellular matrix (dsecm) improves the physical and biological properties of fibrinogen hydrogel for skin bioprinting applications
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466410/
https://www.ncbi.nlm.nih.gov/pubmed/32751101
http://dx.doi.org/10.3390/nano10081484
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