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Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration

The bone-tissue engineering (BTE) field is continuously growing due to a major need for bone substitutes in cases of serious traumas, when the bone tissue has reduced capacity for self-regeneration. So far, graphene oxide (GO)-reinforced natural materials provide satisfactory results for BTE, for bo...

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Autores principales: Vlasceanu, George Mihail, Șelaru, Aida, Dinescu, Sorina, Balta, Cornel, Herman, Hildegard, Gharbia, Sami, Hermenean, Anca, Ionita, Mariana, Costache, Marieta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466474/
https://www.ncbi.nlm.nih.gov/pubmed/32722040
http://dx.doi.org/10.3390/nano10081444
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author Vlasceanu, George Mihail
Șelaru, Aida
Dinescu, Sorina
Balta, Cornel
Herman, Hildegard
Gharbia, Sami
Hermenean, Anca
Ionita, Mariana
Costache, Marieta
author_facet Vlasceanu, George Mihail
Șelaru, Aida
Dinescu, Sorina
Balta, Cornel
Herman, Hildegard
Gharbia, Sami
Hermenean, Anca
Ionita, Mariana
Costache, Marieta
author_sort Vlasceanu, George Mihail
collection PubMed
description The bone-tissue engineering (BTE) field is continuously growing due to a major need for bone substitutes in cases of serious traumas, when the bone tissue has reduced capacity for self-regeneration. So far, graphene oxide (GO)-reinforced natural materials provide satisfactory results for BTE, for both in vitro and in vivo conditions. In this study, we aimed to evaluate the biocompatibility of a new biocomposite consisting of chitosan and fish gelatin crosslinked with genipin and loaded with various concentrations of GO (0.5, 1, 2, 3 wt.%) for prospective BTE applications. Scaffold characterizations revealed a constant swelling degree and good resistance to enzyme degradation. The composites presented a porous structure with pores of similar size, thus mimicking the bone structure. In vitro biocompatibility assays demonstrated an overall beneficial interaction between preosteoblasts, and these particular composites, particularly with 0.5 wt.% GO, reinforced composition. Next, the materials were implanted subcutaneously in 6-week old CD1 mice for in vivo evaluation of biocompatibility and inflammatory activity. Immunohistochemical staining revealed maximal cell infiltration and minimal inflammatory reaction for fish gelatin/chitosan/genipin with 0.5 wt.% GO scaffold, thus demonstrating the best biocompatibility for this particular composition, confirming the in vitro results. This study revealed the potential use of fish gelatin/chitosan GO composites for further implementation in the BTE field.
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spelling pubmed-74664742020-09-14 Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration Vlasceanu, George Mihail Șelaru, Aida Dinescu, Sorina Balta, Cornel Herman, Hildegard Gharbia, Sami Hermenean, Anca Ionita, Mariana Costache, Marieta Nanomaterials (Basel) Article The bone-tissue engineering (BTE) field is continuously growing due to a major need for bone substitutes in cases of serious traumas, when the bone tissue has reduced capacity for self-regeneration. So far, graphene oxide (GO)-reinforced natural materials provide satisfactory results for BTE, for both in vitro and in vivo conditions. In this study, we aimed to evaluate the biocompatibility of a new biocomposite consisting of chitosan and fish gelatin crosslinked with genipin and loaded with various concentrations of GO (0.5, 1, 2, 3 wt.%) for prospective BTE applications. Scaffold characterizations revealed a constant swelling degree and good resistance to enzyme degradation. The composites presented a porous structure with pores of similar size, thus mimicking the bone structure. In vitro biocompatibility assays demonstrated an overall beneficial interaction between preosteoblasts, and these particular composites, particularly with 0.5 wt.% GO, reinforced composition. Next, the materials were implanted subcutaneously in 6-week old CD1 mice for in vivo evaluation of biocompatibility and inflammatory activity. Immunohistochemical staining revealed maximal cell infiltration and minimal inflammatory reaction for fish gelatin/chitosan/genipin with 0.5 wt.% GO scaffold, thus demonstrating the best biocompatibility for this particular composition, confirming the in vitro results. This study revealed the potential use of fish gelatin/chitosan GO composites for further implementation in the BTE field. MDPI 2020-07-24 /pmc/articles/PMC7466474/ /pubmed/32722040 http://dx.doi.org/10.3390/nano10081444 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vlasceanu, George Mihail
Șelaru, Aida
Dinescu, Sorina
Balta, Cornel
Herman, Hildegard
Gharbia, Sami
Hermenean, Anca
Ionita, Mariana
Costache, Marieta
Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title_full Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title_fullStr Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title_full_unstemmed Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title_short Comprehensive Appraisal of Graphene–Oxide Ratio in Porous Biopolymer Hybrids Targeting Bone-Tissue Regeneration
title_sort comprehensive appraisal of graphene–oxide ratio in porous biopolymer hybrids targeting bone-tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466474/
https://www.ncbi.nlm.nih.gov/pubmed/32722040
http://dx.doi.org/10.3390/nano10081444
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