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Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane

Sol-gel-based mesopores allow the entry of target small molecules retained in their cavity and aptamers to bind to target molecules. Herein, sol-gel-based materials are applied to screen-selective aptamers for small molecules, such as pesticides. To enhance the efficiency of aptamer screening using...

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Autores principales: Lim, Eun Seob, Lim, Min-Cheol, Park, Kisang, Lee, Gaeul, Lim, Jeong-A, Woo, Min-Ah, Lee, Nari, Choi, Sung-Wook, Chang, Hyun-Joo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466512/
https://www.ncbi.nlm.nih.gov/pubmed/32764256
http://dx.doi.org/10.3390/nano10081533
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author Lim, Eun Seob
Lim, Min-Cheol
Park, Kisang
Lee, Gaeul
Lim, Jeong-A
Woo, Min-Ah
Lee, Nari
Choi, Sung-Wook
Chang, Hyun-Joo
author_facet Lim, Eun Seob
Lim, Min-Cheol
Park, Kisang
Lee, Gaeul
Lim, Jeong-A
Woo, Min-Ah
Lee, Nari
Choi, Sung-Wook
Chang, Hyun-Joo
author_sort Lim, Eun Seob
collection PubMed
description Sol-gel-based mesopores allow the entry of target small molecules retained in their cavity and aptamers to bind to target molecules. Herein, sol-gel-based materials are applied to screen-selective aptamers for small molecules, such as pesticides. To enhance the efficiency of aptamer screening using a sol-gel, it is necessary to increase the binding surface. In this study, we applied the sol-gel to an anodized aluminum oxide (AAO) membrane, and the morphological features were observed via electron microscopy after spin coating. The binding and elution processes were conducted and confirmed by fluorescence microscopy and polymerase chain reaction. The sol-gel coating on the AAO membrane formed a hollow nanocolumn structure. A diazinon-binding aptamer was bound to the diazinon-containing sol-gel-coated AAO membrane, and the bound aptamer was effectively retrieved from the sol-gel matrix by thermal elution. As a proof of concept, a sol-gel-coated AAO disc was mounted on the edge of a pipette tip, and the feasibility of the prepared platform for the systematic evolution of ligands by exponential enrichment (SELEX) of the aptamer binding was also confirmed. The proposed approach will be applied to an automated SELEX cycle using an automated dispenser, such as a pipetting robot, in the near future.
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spelling pubmed-74665122020-09-14 Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane Lim, Eun Seob Lim, Min-Cheol Park, Kisang Lee, Gaeul Lim, Jeong-A Woo, Min-Ah Lee, Nari Choi, Sung-Wook Chang, Hyun-Joo Nanomaterials (Basel) Article Sol-gel-based mesopores allow the entry of target small molecules retained in their cavity and aptamers to bind to target molecules. Herein, sol-gel-based materials are applied to screen-selective aptamers for small molecules, such as pesticides. To enhance the efficiency of aptamer screening using a sol-gel, it is necessary to increase the binding surface. In this study, we applied the sol-gel to an anodized aluminum oxide (AAO) membrane, and the morphological features were observed via electron microscopy after spin coating. The binding and elution processes were conducted and confirmed by fluorescence microscopy and polymerase chain reaction. The sol-gel coating on the AAO membrane formed a hollow nanocolumn structure. A diazinon-binding aptamer was bound to the diazinon-containing sol-gel-coated AAO membrane, and the bound aptamer was effectively retrieved from the sol-gel matrix by thermal elution. As a proof of concept, a sol-gel-coated AAO disc was mounted on the edge of a pipette tip, and the feasibility of the prepared platform for the systematic evolution of ligands by exponential enrichment (SELEX) of the aptamer binding was also confirmed. The proposed approach will be applied to an automated SELEX cycle using an automated dispenser, such as a pipetting robot, in the near future. MDPI 2020-08-05 /pmc/articles/PMC7466512/ /pubmed/32764256 http://dx.doi.org/10.3390/nano10081533 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lim, Eun Seob
Lim, Min-Cheol
Park, Kisang
Lee, Gaeul
Lim, Jeong-A
Woo, Min-Ah
Lee, Nari
Choi, Sung-Wook
Chang, Hyun-Joo
Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title_full Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title_fullStr Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title_full_unstemmed Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title_short Selective Binding and Elution of Aptamers for Pesticides Based on Sol-Gel-Coated Nanoporous Anodized Aluminum Oxide Membrane
title_sort selective binding and elution of aptamers for pesticides based on sol-gel-coated nanoporous anodized aluminum oxide membrane
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466512/
https://www.ncbi.nlm.nih.gov/pubmed/32764256
http://dx.doi.org/10.3390/nano10081533
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