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Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials

Cancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompa...

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Autores principales: Lopes, Joana, Coelho, João Miguel Pinto, Vieira, Pedro Manuel Cardoso, Viana, Ana Silveira, Gaspar, Maria Manuela, Reis, Catarina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466595/
https://www.ncbi.nlm.nih.gov/pubmed/32764377
http://dx.doi.org/10.3390/nano10081536
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author Lopes, Joana
Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina
author_facet Lopes, Joana
Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina
author_sort Lopes, Joana
collection PubMed
description Cancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompatible gold nanoparticles (GNPs) coated with hyaluronic acid and oleic acid were prepared and characterized in terms of size, morphology and cytotoxicity in the presence of Saccharomyces cerevisiae, and two cell lines, the keratinocytes (healthy skin cells, HaCat) and the melanoma cells (B16F10). Results showed that these GNPs absorb within the near-infrared region (750–1400 nm), in the optical therapeutic window (from 650 to 1300 nm), in contrast to other commercial gold nanoparticles, which enables light to penetrate into deep skin layers. A laser emitting in this region was applied and its effect also analyzed. The coated GNPs showed a spherical morphology with a mean size of 297 nm without cytotoxic effects towards yeast and tested cell lines. Nevertheless, after laser irradiation, a reduction of 20% in B16F10 cell line viability was observed. In summary, this work appears to be a promising strategy for the treatment of non-metastatic melanoma or other superficial tumors.
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spelling pubmed-74665952020-09-14 Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials Lopes, Joana Coelho, João Miguel Pinto Vieira, Pedro Manuel Cardoso Viana, Ana Silveira Gaspar, Maria Manuela Reis, Catarina Nanomaterials (Basel) Article Cancer like melanoma is a complex disease, for which standard therapies have significant adverse side effects that in most cases are ineffective and highly unspecific. Thus, a new paradigm has come with the need of achieving alternative (less invasive) and effective therapies. In this work, biocompatible gold nanoparticles (GNPs) coated with hyaluronic acid and oleic acid were prepared and characterized in terms of size, morphology and cytotoxicity in the presence of Saccharomyces cerevisiae, and two cell lines, the keratinocytes (healthy skin cells, HaCat) and the melanoma cells (B16F10). Results showed that these GNPs absorb within the near-infrared region (750–1400 nm), in the optical therapeutic window (from 650 to 1300 nm), in contrast to other commercial gold nanoparticles, which enables light to penetrate into deep skin layers. A laser emitting in this region was applied and its effect also analyzed. The coated GNPs showed a spherical morphology with a mean size of 297 nm without cytotoxic effects towards yeast and tested cell lines. Nevertheless, after laser irradiation, a reduction of 20% in B16F10 cell line viability was observed. In summary, this work appears to be a promising strategy for the treatment of non-metastatic melanoma or other superficial tumors. MDPI 2020-08-05 /pmc/articles/PMC7466595/ /pubmed/32764377 http://dx.doi.org/10.3390/nano10081536 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lopes, Joana
Coelho, João Miguel Pinto
Vieira, Pedro Manuel Cardoso
Viana, Ana Silveira
Gaspar, Maria Manuela
Reis, Catarina
Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_full Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_fullStr Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_full_unstemmed Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_short Preliminary Assays towards Melanoma Cells Using Phototherapy with Gold-Based Nanomaterials
title_sort preliminary assays towards melanoma cells using phototherapy with gold-based nanomaterials
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466595/
https://www.ncbi.nlm.nih.gov/pubmed/32764377
http://dx.doi.org/10.3390/nano10081536
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