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Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities

Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmi...

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Autores principales: Prussing, Catharine, Snavely, Emily A., Singh, Navjot, Lapierre, Pascal, Lasek-Nesselquist, Erica, Mitchell, Kara, Haas, Wolfgang, Owsiak, Rita, Nazarian, Elizabeth, Musser, Kimberlee A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466660/
https://www.ncbi.nlm.nih.gov/pubmed/32973725
http://dx.doi.org/10.3389/fmicb.2020.02007
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author Prussing, Catharine
Snavely, Emily A.
Singh, Navjot
Lapierre, Pascal
Lasek-Nesselquist, Erica
Mitchell, Kara
Haas, Wolfgang
Owsiak, Rita
Nazarian, Elizabeth
Musser, Kimberlee A.
author_facet Prussing, Catharine
Snavely, Emily A.
Singh, Navjot
Lapierre, Pascal
Lasek-Nesselquist, Erica
Mitchell, Kara
Haas, Wolfgang
Owsiak, Rita
Nazarian, Elizabeth
Musser, Kimberlee A.
author_sort Prussing, Catharine
collection PubMed
description Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmitted between bacteria of the same or different species. Here we describe the results of an epidemiological investigation of KPC-producing bacteria at two healthcare facilities. Using a combination of short-read and long-read whole-genome sequencing, we identified an identical 44 kilobase plasmid carrying the bla(KPC–2) gene in four bacterial isolates belonging to three different species (Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli). The isolates in this investigation were collected from patients who were epidemiologically linked in a region in which KPC was uncommon, suggesting that the antibiotic resistance plasmid was transmitted between these bacterial species. This investigation highlights the importance of long-read sequencing in investigating the relatedness of bacterial plasmids, and in elucidating potential plasmid-mediated outbreaks caused by antibiotic resistant bacteria.
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spelling pubmed-74666602020-09-23 Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities Prussing, Catharine Snavely, Emily A. Singh, Navjot Lapierre, Pascal Lasek-Nesselquist, Erica Mitchell, Kara Haas, Wolfgang Owsiak, Rita Nazarian, Elizabeth Musser, Kimberlee A. Front Microbiol Microbiology Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmitted between bacteria of the same or different species. Here we describe the results of an epidemiological investigation of KPC-producing bacteria at two healthcare facilities. Using a combination of short-read and long-read whole-genome sequencing, we identified an identical 44 kilobase plasmid carrying the bla(KPC–2) gene in four bacterial isolates belonging to three different species (Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli). The isolates in this investigation were collected from patients who were epidemiologically linked in a region in which KPC was uncommon, suggesting that the antibiotic resistance plasmid was transmitted between these bacterial species. This investigation highlights the importance of long-read sequencing in investigating the relatedness of bacterial plasmids, and in elucidating potential plasmid-mediated outbreaks caused by antibiotic resistant bacteria. Frontiers Media S.A. 2020-08-19 /pmc/articles/PMC7466660/ /pubmed/32973725 http://dx.doi.org/10.3389/fmicb.2020.02007 Text en Copyright © 2020 Prussing, Snavely, Singh, Lapierre, Lasek-Nesselquist, Mitchell, Haas, Owsiak, Nazarian and Musser. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Prussing, Catharine
Snavely, Emily A.
Singh, Navjot
Lapierre, Pascal
Lasek-Nesselquist, Erica
Mitchell, Kara
Haas, Wolfgang
Owsiak, Rita
Nazarian, Elizabeth
Musser, Kimberlee A.
Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title_full Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title_fullStr Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title_full_unstemmed Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title_short Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
title_sort nanopore minion sequencing reveals possible transfer of bla(kpc–2) plasmid across bacterial species in two healthcare facilities
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466660/
https://www.ncbi.nlm.nih.gov/pubmed/32973725
http://dx.doi.org/10.3389/fmicb.2020.02007
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