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Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities
Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466660/ https://www.ncbi.nlm.nih.gov/pubmed/32973725 http://dx.doi.org/10.3389/fmicb.2020.02007 |
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author | Prussing, Catharine Snavely, Emily A. Singh, Navjot Lapierre, Pascal Lasek-Nesselquist, Erica Mitchell, Kara Haas, Wolfgang Owsiak, Rita Nazarian, Elizabeth Musser, Kimberlee A. |
author_facet | Prussing, Catharine Snavely, Emily A. Singh, Navjot Lapierre, Pascal Lasek-Nesselquist, Erica Mitchell, Kara Haas, Wolfgang Owsiak, Rita Nazarian, Elizabeth Musser, Kimberlee A. |
author_sort | Prussing, Catharine |
collection | PubMed |
description | Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmitted between bacteria of the same or different species. Here we describe the results of an epidemiological investigation of KPC-producing bacteria at two healthcare facilities. Using a combination of short-read and long-read whole-genome sequencing, we identified an identical 44 kilobase plasmid carrying the bla(KPC–2) gene in four bacterial isolates belonging to three different species (Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli). The isolates in this investigation were collected from patients who were epidemiologically linked in a region in which KPC was uncommon, suggesting that the antibiotic resistance plasmid was transmitted between these bacterial species. This investigation highlights the importance of long-read sequencing in investigating the relatedness of bacterial plasmids, and in elucidating potential plasmid-mediated outbreaks caused by antibiotic resistant bacteria. |
format | Online Article Text |
id | pubmed-7466660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74666602020-09-23 Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities Prussing, Catharine Snavely, Emily A. Singh, Navjot Lapierre, Pascal Lasek-Nesselquist, Erica Mitchell, Kara Haas, Wolfgang Owsiak, Rita Nazarian, Elizabeth Musser, Kimberlee A. Front Microbiol Microbiology Carbapenemase-producing Enterobacteriaceae are a major threat to global public health. Klebsiella pneumoniae carbapenemase (KPC) is the most commonly identified carbapenemase in the United States and is frequently found on mobile genetic elements including plasmids, which can be horizontally transmitted between bacteria of the same or different species. Here we describe the results of an epidemiological investigation of KPC-producing bacteria at two healthcare facilities. Using a combination of short-read and long-read whole-genome sequencing, we identified an identical 44 kilobase plasmid carrying the bla(KPC–2) gene in four bacterial isolates belonging to three different species (Citrobacter freundii, Klebsiella pneumoniae, and Escherichia coli). The isolates in this investigation were collected from patients who were epidemiologically linked in a region in which KPC was uncommon, suggesting that the antibiotic resistance plasmid was transmitted between these bacterial species. This investigation highlights the importance of long-read sequencing in investigating the relatedness of bacterial plasmids, and in elucidating potential plasmid-mediated outbreaks caused by antibiotic resistant bacteria. Frontiers Media S.A. 2020-08-19 /pmc/articles/PMC7466660/ /pubmed/32973725 http://dx.doi.org/10.3389/fmicb.2020.02007 Text en Copyright © 2020 Prussing, Snavely, Singh, Lapierre, Lasek-Nesselquist, Mitchell, Haas, Owsiak, Nazarian and Musser. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Prussing, Catharine Snavely, Emily A. Singh, Navjot Lapierre, Pascal Lasek-Nesselquist, Erica Mitchell, Kara Haas, Wolfgang Owsiak, Rita Nazarian, Elizabeth Musser, Kimberlee A. Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title | Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title_full | Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title_fullStr | Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title_full_unstemmed | Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title_short | Nanopore MinION Sequencing Reveals Possible Transfer of bla(KPC–2) Plasmid Across Bacterial Species in Two Healthcare Facilities |
title_sort | nanopore minion sequencing reveals possible transfer of bla(kpc–2) plasmid across bacterial species in two healthcare facilities |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466660/ https://www.ncbi.nlm.nih.gov/pubmed/32973725 http://dx.doi.org/10.3389/fmicb.2020.02007 |
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