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Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes

In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) fo...

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Autores principales: Pelin, Marco, Lin, Hazel, Gazzi, Arianna, Sosa, Silvio, Ponti, Cristina, Ortega, Amaya, Zurutuza, Amaia, Vázquez, Ester, Prato, Maurizio, Tubaro, Aurelia, Bianco, Alberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466707/
https://www.ncbi.nlm.nih.gov/pubmed/32824126
http://dx.doi.org/10.3390/nano10081602
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author Pelin, Marco
Lin, Hazel
Gazzi, Arianna
Sosa, Silvio
Ponti, Cristina
Ortega, Amaya
Zurutuza, Amaia
Vázquez, Ester
Prato, Maurizio
Tubaro, Aurelia
Bianco, Alberto
author_facet Pelin, Marco
Lin, Hazel
Gazzi, Arianna
Sosa, Silvio
Ponti, Cristina
Ortega, Amaya
Zurutuza, Amaia
Vázquez, Ester
Prato, Maurizio
Tubaro, Aurelia
Bianco, Alberto
author_sort Pelin, Marco
collection PubMed
description In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices.
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spelling pubmed-74667072020-09-14 Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes Pelin, Marco Lin, Hazel Gazzi, Arianna Sosa, Silvio Ponti, Cristina Ortega, Amaya Zurutuza, Amaia Vázquez, Ester Prato, Maurizio Tubaro, Aurelia Bianco, Alberto Nanomaterials (Basel) Article In the frame of graphene-based material (GBM) hazard characterization, particular attention should be given to the cutaneous effects. Hence, this study investigates if HaCaT skin keratinocytes exposed to high concentrations of few-layer graphene (FLG) or partially dehydrated graphene oxide (d-GO) for a short time can recover from the cytotoxic insult, measured by means of cell viability, mitochondrial damage and oxidative stress, after GBM removal from the cell medium. When compared to 24 or 72 h continuous exposure, recovery experiments suggest that the cytotoxicity induced by 24 h exposure to GBM is only partially recovered after 48 h culture in GBM-free medium. This partial recovery, higher for FLG as compared to GO, is not mediated by autophagy and could be the consequence of GBM internalization into cells. The ability of GBMs to be internalized inside keratinocytes together with the partial reversibility of the cellular damage is important in assessing the risk associated with skin exposure to GBM-containing devices. MDPI 2020-08-15 /pmc/articles/PMC7466707/ /pubmed/32824126 http://dx.doi.org/10.3390/nano10081602 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pelin, Marco
Lin, Hazel
Gazzi, Arianna
Sosa, Silvio
Ponti, Cristina
Ortega, Amaya
Zurutuza, Amaia
Vázquez, Ester
Prato, Maurizio
Tubaro, Aurelia
Bianco, Alberto
Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title_full Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title_fullStr Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title_full_unstemmed Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title_short Partial Reversibility of the Cytotoxic Effect Induced by Graphene-Based Materials in Skin Keratinocytes
title_sort partial reversibility of the cytotoxic effect induced by graphene-based materials in skin keratinocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466707/
https://www.ncbi.nlm.nih.gov/pubmed/32824126
http://dx.doi.org/10.3390/nano10081602
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