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Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus

Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. The fungi rich in SM production are taxonomically concentrated in the subkingdom Dikarya, which comprises the phyla Ascomycota...

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Autores principales: Tabima, Javier F., Trautman, Ian A., Chang, Ying, Wang, Yan, Mondo, Stephen, Kuo, Alan, Salamov, Asaf, Grigoriev, Igor V., Stajich, Jason E., Spatafora, Joseph W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466969/
https://www.ncbi.nlm.nih.gov/pubmed/32727924
http://dx.doi.org/10.1534/g3.120.401516
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author Tabima, Javier F.
Trautman, Ian A.
Chang, Ying
Wang, Yan
Mondo, Stephen
Kuo, Alan
Salamov, Asaf
Grigoriev, Igor V.
Stajich, Jason E.
Spatafora, Joseph W.
author_facet Tabima, Javier F.
Trautman, Ian A.
Chang, Ying
Wang, Yan
Mondo, Stephen
Kuo, Alan
Salamov, Asaf
Grigoriev, Igor V.
Stajich, Jason E.
Spatafora, Joseph W.
author_sort Tabima, Javier F.
collection PubMed
description Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. The fungi rich in SM production are taxonomically concentrated in the subkingdom Dikarya, which comprises the phyla Ascomycota and Basidiomycota. Here, we explore the potential for SM production in Mucoromycota and Zoopagomycota, two phyla of nonflagellated fungi that are not members of Dikarya, by predicting and identifying core genes and gene clusters involved in SM. The majority of non-Dikarya have few genes and gene clusters involved in SM production except for the amphibian gut symbionts in the genus Basidiobolus. Basidiobolus genomes exhibit an enrichment of SM genes involved in siderophore, surfactin-like, and terpene cyclase production, all these with evidence of constitutive gene expression. Gene expression and chemical assays also confirm that Basidiobolus has significant siderophore activity. The expansion of SMs in Basidiobolus are partially due to horizontal gene transfer from bacteria, likely as a consequence of its ecology as an amphibian gut endosymbiont.
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spelling pubmed-74669692020-09-14 Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus Tabima, Javier F. Trautman, Ian A. Chang, Ying Wang, Yan Mondo, Stephen Kuo, Alan Salamov, Asaf Grigoriev, Igor V. Stajich, Jason E. Spatafora, Joseph W. G3 (Bethesda) Investigations Research into secondary metabolism (SM) production by fungi has resulted in the discovery of diverse, biologically active compounds with significant medicinal applications. The fungi rich in SM production are taxonomically concentrated in the subkingdom Dikarya, which comprises the phyla Ascomycota and Basidiomycota. Here, we explore the potential for SM production in Mucoromycota and Zoopagomycota, two phyla of nonflagellated fungi that are not members of Dikarya, by predicting and identifying core genes and gene clusters involved in SM. The majority of non-Dikarya have few genes and gene clusters involved in SM production except for the amphibian gut symbionts in the genus Basidiobolus. Basidiobolus genomes exhibit an enrichment of SM genes involved in siderophore, surfactin-like, and terpene cyclase production, all these with evidence of constitutive gene expression. Gene expression and chemical assays also confirm that Basidiobolus has significant siderophore activity. The expansion of SMs in Basidiobolus are partially due to horizontal gene transfer from bacteria, likely as a consequence of its ecology as an amphibian gut endosymbiont. Genetics Society of America 2020-07-29 /pmc/articles/PMC7466969/ /pubmed/32727924 http://dx.doi.org/10.1534/g3.120.401516 Text en Copyright © 2020 Tabima et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Tabima, Javier F.
Trautman, Ian A.
Chang, Ying
Wang, Yan
Mondo, Stephen
Kuo, Alan
Salamov, Asaf
Grigoriev, Igor V.
Stajich, Jason E.
Spatafora, Joseph W.
Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title_full Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title_fullStr Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title_full_unstemmed Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title_short Phylogenomic Analyses of Non-Dikarya Fungi Supports Horizontal Gene Transfer Driving Diversification of Secondary Metabolism in the Amphibian Gastrointestinal Symbiont, Basidiobolus
title_sort phylogenomic analyses of non-dikarya fungi supports horizontal gene transfer driving diversification of secondary metabolism in the amphibian gastrointestinal symbiont, basidiobolus
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7466969/
https://www.ncbi.nlm.nih.gov/pubmed/32727924
http://dx.doi.org/10.1534/g3.120.401516
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