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A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions

The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The col...

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Autores principales: Kim, Dae-Kyum, Knapp, Jennifer J., Kuang, Da, Chawla, Aditya, Cassonnet, Patricia, Lee, Hunsang, Sheykhkarimli, Dayag, Samavarchi-Tehrani, Payman, Abdouni, Hala, Rayhan, Ashyad, Li, Roujia, Pogoutse, Oxana, Coyaud, Étienne, van der Werf, Sylvie, Demeret, Caroline, Gingras, Anne-Claude, Taipale, Mikko, Raught, Brian, Jacob, Yves, Roth, Frederick P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467003/
https://www.ncbi.nlm.nih.gov/pubmed/32763951
http://dx.doi.org/10.1534/g3.120.401554
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author Kim, Dae-Kyum
Knapp, Jennifer J.
Kuang, Da
Chawla, Aditya
Cassonnet, Patricia
Lee, Hunsang
Sheykhkarimli, Dayag
Samavarchi-Tehrani, Payman
Abdouni, Hala
Rayhan, Ashyad
Li, Roujia
Pogoutse, Oxana
Coyaud, Étienne
van der Werf, Sylvie
Demeret, Caroline
Gingras, Anne-Claude
Taipale, Mikko
Raught, Brian
Jacob, Yves
Roth, Frederick P.
author_facet Kim, Dae-Kyum
Knapp, Jennifer J.
Kuang, Da
Chawla, Aditya
Cassonnet, Patricia
Lee, Hunsang
Sheykhkarimli, Dayag
Samavarchi-Tehrani, Payman
Abdouni, Hala
Rayhan, Ashyad
Li, Roujia
Pogoutse, Oxana
Coyaud, Étienne
van der Werf, Sylvie
Demeret, Caroline
Gingras, Anne-Claude
Taipale, Mikko
Raught, Brian
Jacob, Yves
Roth, Frederick P.
author_sort Kim, Dae-Kyum
collection PubMed
description The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.
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spelling pubmed-74670032020-09-14 A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions Kim, Dae-Kyum Knapp, Jennifer J. Kuang, Da Chawla, Aditya Cassonnet, Patricia Lee, Hunsang Sheykhkarimli, Dayag Samavarchi-Tehrani, Payman Abdouni, Hala Rayhan, Ashyad Li, Roujia Pogoutse, Oxana Coyaud, Étienne van der Werf, Sylvie Demeret, Caroline Gingras, Anne-Claude Taipale, Mikko Raught, Brian Jacob, Yves Roth, Frederick P. G3 (Bethesda) Investigations The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it. Genetics Society of America 2020-08-06 /pmc/articles/PMC7467003/ /pubmed/32763951 http://dx.doi.org/10.1534/g3.120.401554 Text en Copyright © 2020 Kim et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Kim, Dae-Kyum
Knapp, Jennifer J.
Kuang, Da
Chawla, Aditya
Cassonnet, Patricia
Lee, Hunsang
Sheykhkarimli, Dayag
Samavarchi-Tehrani, Payman
Abdouni, Hala
Rayhan, Ashyad
Li, Roujia
Pogoutse, Oxana
Coyaud, Étienne
van der Werf, Sylvie
Demeret, Caroline
Gingras, Anne-Claude
Taipale, Mikko
Raught, Brian
Jacob, Yves
Roth, Frederick P.
A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title_full A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title_fullStr A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title_full_unstemmed A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title_short A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions
title_sort comprehensive, flexible collection of sars-cov-2 coding regions
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467003/
https://www.ncbi.nlm.nih.gov/pubmed/32763951
http://dx.doi.org/10.1534/g3.120.401554
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