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Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

DNA assembly is an integral part of modern synthetic biology, as intricate genetic engineering projects require robust molecular cloning workflows. Golden Gate assembly is a frequently employed DNA assembly methodology that utilizes a Type IIS restriction enzyme and a DNA ligase to generate recombin...

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Detalles Bibliográficos
Autores principales:	Pryor, John M., Potapov, Vladimir, Kucera, Rebecca B., Bilotti, Katharina, Cantor, Eric J., Lohman, Gregory J. S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2020
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467295/ https://www.ncbi.nlm.nih.gov/pubmed/32877448 http://dx.doi.org/10.1371/journal.pone.0238592

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