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Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments

Hard X-ray nanotomography enables 3D investigations of a wide range of samples with high resolution (<100 nm) with both synchrotron-based and laboratory-based setups. However, the advantage of synchrotron-based setups is the high flux, enabling time resolution, which cannot be achieved at laborat...

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Autores principales: Flenner, Silja, Storm, Malte, Kubec, Adam, Longo, Elena, Döring, Florian, Pelt, Daniël M., David, Christian, Müller, Martin, Greving, Imke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467338/
https://www.ncbi.nlm.nih.gov/pubmed/32876609
http://dx.doi.org/10.1107/S1600577520007407
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author Flenner, Silja
Storm, Malte
Kubec, Adam
Longo, Elena
Döring, Florian
Pelt, Daniël M.
David, Christian
Müller, Martin
Greving, Imke
author_facet Flenner, Silja
Storm, Malte
Kubec, Adam
Longo, Elena
Döring, Florian
Pelt, Daniël M.
David, Christian
Müller, Martin
Greving, Imke
author_sort Flenner, Silja
collection PubMed
description Hard X-ray nanotomography enables 3D investigations of a wide range of samples with high resolution (<100 nm) with both synchrotron-based and laboratory-based setups. However, the advantage of synchrotron-based setups is the high flux, enabling time resolution, which cannot be achieved at laboratory sources. Here, the nanotomography setup at the imaging beamline P05 at PETRA III is presented, which offers high time resolution not only in absorption but for the first time also in Zernike phase contrast. Two test samples are used to evaluate the image quality in both contrast modalities based on the quantitative analysis of contrast-to-noise ratio (CNR) and spatial resolution. High-quality scans can be recorded in 15 min and fast scans down to 3 min are also possible without significant loss of image quality. At scan times well below 3 min, the CNR values decrease significantly and classical image-filtering techniques reach their limitation. A machine-learning approach shows promising results, enabling acquisition of a full tomography in only 6 s. Overall, the transmission X-ray microscopy instrument offers high temporal resolution in absorption and Zernike phase contrast, enabling in situ experiments at the beamline.
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spelling pubmed-74673382020-09-15 Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments Flenner, Silja Storm, Malte Kubec, Adam Longo, Elena Döring, Florian Pelt, Daniël M. David, Christian Müller, Martin Greving, Imke J Synchrotron Radiat Research Papers Hard X-ray nanotomography enables 3D investigations of a wide range of samples with high resolution (<100 nm) with both synchrotron-based and laboratory-based setups. However, the advantage of synchrotron-based setups is the high flux, enabling time resolution, which cannot be achieved at laboratory sources. Here, the nanotomography setup at the imaging beamline P05 at PETRA III is presented, which offers high time resolution not only in absorption but for the first time also in Zernike phase contrast. Two test samples are used to evaluate the image quality in both contrast modalities based on the quantitative analysis of contrast-to-noise ratio (CNR) and spatial resolution. High-quality scans can be recorded in 15 min and fast scans down to 3 min are also possible without significant loss of image quality. At scan times well below 3 min, the CNR values decrease significantly and classical image-filtering techniques reach their limitation. A machine-learning approach shows promising results, enabling acquisition of a full tomography in only 6 s. Overall, the transmission X-ray microscopy instrument offers high temporal resolution in absorption and Zernike phase contrast, enabling in situ experiments at the beamline. International Union of Crystallography 2020-07-30 /pmc/articles/PMC7467338/ /pubmed/32876609 http://dx.doi.org/10.1107/S1600577520007407 Text en © Flenner et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Flenner, Silja
Storm, Malte
Kubec, Adam
Longo, Elena
Döring, Florian
Pelt, Daniël M.
David, Christian
Müller, Martin
Greving, Imke
Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title_full Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title_fullStr Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title_full_unstemmed Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title_short Pushing the temporal resolution in absorption and Zernike phase contrast nanotomography: enabling fast in situ experiments
title_sort pushing the temporal resolution in absorption and zernike phase contrast nanotomography: enabling fast in situ experiments
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467338/
https://www.ncbi.nlm.nih.gov/pubmed/32876609
http://dx.doi.org/10.1107/S1600577520007407
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