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Current status and future opportunities for serial crystallography at MAX IV Laboratory

Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radi...

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Autores principales: Shilova, Anastasya, Lebrette, Hugo, Aurelius, Oskar, Nan, Jie, Welin, Martin, Kovacic, Rebeka, Ghosh, Swagatha, Safari, Cecilia, Friel, Ross J., Milas, Mirko, Matej, Zdenek, Högbom, Martin, Brändén, Gisela, Kloos, Marco, Shoeman, Robert L., Doak, Bruce, Ursby, Thomas, Håkansson, Maria, Logan, Derek T., Mueller, Uwe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467353/
https://www.ncbi.nlm.nih.gov/pubmed/32876583
http://dx.doi.org/10.1107/S1600577520008735
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author Shilova, Anastasya
Lebrette, Hugo
Aurelius, Oskar
Nan, Jie
Welin, Martin
Kovacic, Rebeka
Ghosh, Swagatha
Safari, Cecilia
Friel, Ross J.
Milas, Mirko
Matej, Zdenek
Högbom, Martin
Brändén, Gisela
Kloos, Marco
Shoeman, Robert L.
Doak, Bruce
Ursby, Thomas
Håkansson, Maria
Logan, Derek T.
Mueller, Uwe
author_facet Shilova, Anastasya
Lebrette, Hugo
Aurelius, Oskar
Nan, Jie
Welin, Martin
Kovacic, Rebeka
Ghosh, Swagatha
Safari, Cecilia
Friel, Ross J.
Milas, Mirko
Matej, Zdenek
Högbom, Martin
Brändén, Gisela
Kloos, Marco
Shoeman, Robert L.
Doak, Bruce
Ursby, Thomas
Håkansson, Maria
Logan, Derek T.
Mueller, Uwe
author_sort Shilova, Anastasya
collection PubMed
description Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports – silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed.
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spelling pubmed-74673532020-09-15 Current status and future opportunities for serial crystallography at MAX IV Laboratory Shilova, Anastasya Lebrette, Hugo Aurelius, Oskar Nan, Jie Welin, Martin Kovacic, Rebeka Ghosh, Swagatha Safari, Cecilia Friel, Ross J. Milas, Mirko Matej, Zdenek Högbom, Martin Brändén, Gisela Kloos, Marco Shoeman, Robert L. Doak, Bruce Ursby, Thomas Håkansson, Maria Logan, Derek T. Mueller, Uwe J Synchrotron Radiat Research Papers Over the last decade, serial crystallography, a method to collect complete diffraction datasets from a large number of microcrystals delivered and exposed to an X-ray beam in random orientations at room temperature, has been successfully implemented at X-ray free-electron lasers and synchrotron radiation facility beamlines. This development relies on a growing variety of sample presentation methods, including different fixed target supports, injection methods using gas-dynamic virtual-nozzle injectors and high-viscosity extrusion injectors, and acoustic levitation of droplets, each with unique requirements. In comparison with X-ray free-electron lasers, increased beam time availability makes synchrotron facilities very attractive to perform serial synchrotron X-ray crystallography (SSX) experiments. Within this work, the possibilities to perform SSX at BioMAX, the first macromolecular crystallography beamline at  MAX IV Laboratory in Lund, Sweden, are described, together with case studies from the SSX user program: an implementation of a high-viscosity extrusion injector to perform room temperature serial crystallography at BioMAX using two solid supports – silicon nitride membranes (Silson, UK) and XtalTool (Jena Bioscience, Germany). Future perspectives for the dedicated serial crystallography beamline MicroMAX at MAX IV Laboratory, which will provide parallel and intense micrometre-sized X-ray beams, are discussed. International Union of Crystallography 2020-08-21 /pmc/articles/PMC7467353/ /pubmed/32876583 http://dx.doi.org/10.1107/S1600577520008735 Text en © Shilova et al. 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Papers
Shilova, Anastasya
Lebrette, Hugo
Aurelius, Oskar
Nan, Jie
Welin, Martin
Kovacic, Rebeka
Ghosh, Swagatha
Safari, Cecilia
Friel, Ross J.
Milas, Mirko
Matej, Zdenek
Högbom, Martin
Brändén, Gisela
Kloos, Marco
Shoeman, Robert L.
Doak, Bruce
Ursby, Thomas
Håkansson, Maria
Logan, Derek T.
Mueller, Uwe
Current status and future opportunities for serial crystallography at MAX IV Laboratory
title Current status and future opportunities for serial crystallography at MAX IV Laboratory
title_full Current status and future opportunities for serial crystallography at MAX IV Laboratory
title_fullStr Current status and future opportunities for serial crystallography at MAX IV Laboratory
title_full_unstemmed Current status and future opportunities for serial crystallography at MAX IV Laboratory
title_short Current status and future opportunities for serial crystallography at MAX IV Laboratory
title_sort current status and future opportunities for serial crystallography at max iv laboratory
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467353/
https://www.ncbi.nlm.nih.gov/pubmed/32876583
http://dx.doi.org/10.1107/S1600577520008735
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