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Bipolar Corona Discharge-Based Charge Equilibration for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analysis of Bio- and Polymer Nanoparticles
[Image: see text] Separation of polydisperse, single-charged analytes in the nanometer size range in a high laminar sheath flow of particle-free ambient air and a tunable electric field based on the respective particle electrophoretic mobility diameter (EMD) can be achieved via gas-phase electrophor...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical
Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467421/ https://www.ncbi.nlm.nih.gov/pubmed/32519840 http://dx.doi.org/10.1021/acs.analchem.0c01904 |
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author | Weiss, Victor U. Frank, Johannes Piplits, Kurt Szymanski, Wladyslaw W. Allmaier, Günter |
author_facet | Weiss, Victor U. Frank, Johannes Piplits, Kurt Szymanski, Wladyslaw W. Allmaier, Günter |
author_sort | Weiss, Victor U. |
collection | PubMed |
description | [Image: see text] Separation of polydisperse, single-charged analytes in the nanometer size range in a high laminar sheath flow of particle-free ambient air and a tunable electric field based on the respective particle electrophoretic mobility diameter (EMD) can be achieved via gas-phase electrophoresis. In order to transfer analytes from a volatile electrolyte solution to the gas-phase as a single-charged species, a nano electrospray (nES) process followed by drying of nanodroplets and charge conditioning reaching Boltzmann charge equilibrium is a necessary prerequisite. In the case of a so-called nES gas-phase electrophoretic mobility molecular analyzer (nES GEMMA, also known as nES differential mobility analyzer, nES DMA), charge equilibration is based on bionanoparticle interaction with a bipolar atmosphere induced, e.g., by a radioactive α-particle emitter like (210)Po. It was the aim of our investigation to examine whether such a radioactive source can be easily replaced in the same nES housing by a nonradioactive one, i.e., by an AC corona discharge unit. The latter would be significantly easier to handle when compared to radioactive material in laboratory day-to-day business, waste disposal, as well as regulatory confinements. Indeed, we were able to combine a standard nES unit of our nES GEMMA instrument with a commercially available AC corona discharge device in a novel setup via an adapter. Our results show that this replacement yields very good results for a number of chemically different nanoparticles, an exemplary protein, a noncovalent protein complex, a virus-like particle, a polymer, and a liposome sample, when compared to a (210)Po based bipolar charge equilibration device. |
format | Online Article Text |
id | pubmed-7467421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74674212020-09-03 Bipolar Corona Discharge-Based Charge Equilibration for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analysis of Bio- and Polymer Nanoparticles Weiss, Victor U. Frank, Johannes Piplits, Kurt Szymanski, Wladyslaw W. Allmaier, Günter Anal Chem [Image: see text] Separation of polydisperse, single-charged analytes in the nanometer size range in a high laminar sheath flow of particle-free ambient air and a tunable electric field based on the respective particle electrophoretic mobility diameter (EMD) can be achieved via gas-phase electrophoresis. In order to transfer analytes from a volatile electrolyte solution to the gas-phase as a single-charged species, a nano electrospray (nES) process followed by drying of nanodroplets and charge conditioning reaching Boltzmann charge equilibrium is a necessary prerequisite. In the case of a so-called nES gas-phase electrophoretic mobility molecular analyzer (nES GEMMA, also known as nES differential mobility analyzer, nES DMA), charge equilibration is based on bionanoparticle interaction with a bipolar atmosphere induced, e.g., by a radioactive α-particle emitter like (210)Po. It was the aim of our investigation to examine whether such a radioactive source can be easily replaced in the same nES housing by a nonradioactive one, i.e., by an AC corona discharge unit. The latter would be significantly easier to handle when compared to radioactive material in laboratory day-to-day business, waste disposal, as well as regulatory confinements. Indeed, we were able to combine a standard nES unit of our nES GEMMA instrument with a commercially available AC corona discharge device in a novel setup via an adapter. Our results show that this replacement yields very good results for a number of chemically different nanoparticles, an exemplary protein, a noncovalent protein complex, a virus-like particle, a polymer, and a liposome sample, when compared to a (210)Po based bipolar charge equilibration device. American Chemical Society 2020-06-10 2020-07-07 /pmc/articles/PMC7467421/ /pubmed/32519840 http://dx.doi.org/10.1021/acs.analchem.0c01904 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Attribution (CC-BY) License (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html) , which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. |
spellingShingle | Weiss, Victor U. Frank, Johannes Piplits, Kurt Szymanski, Wladyslaw W. Allmaier, Günter Bipolar Corona Discharge-Based Charge Equilibration for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analysis of Bio- and Polymer Nanoparticles |
title | Bipolar Corona Discharge-Based Charge Equilibration
for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular
Analysis of Bio- and Polymer Nanoparticles |
title_full | Bipolar Corona Discharge-Based Charge Equilibration
for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular
Analysis of Bio- and Polymer Nanoparticles |
title_fullStr | Bipolar Corona Discharge-Based Charge Equilibration
for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular
Analysis of Bio- and Polymer Nanoparticles |
title_full_unstemmed | Bipolar Corona Discharge-Based Charge Equilibration
for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular
Analysis of Bio- and Polymer Nanoparticles |
title_short | Bipolar Corona Discharge-Based Charge Equilibration
for Nano Electrospray Gas-Phase Electrophoretic Mobility Molecular
Analysis of Bio- and Polymer Nanoparticles |
title_sort | bipolar corona discharge-based charge equilibration
for nano electrospray gas-phase electrophoretic mobility molecular
analysis of bio- and polymer nanoparticles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467421/ https://www.ncbi.nlm.nih.gov/pubmed/32519840 http://dx.doi.org/10.1021/acs.analchem.0c01904 |
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