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Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm

This study evaluated the antibacterial effects of short-time exposure of surface pre-reacted glass-ionomer (S-PRG) eluate on oral microcosm biofilm. Biofilms were treated with an S-PRG eluate at different concentrations (25%, 50%, and 100%), distilled water (DW), and 0.1% chlorhexidine (CHX) twice a...

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Autores principales: Kim, Hyo-Jung, Cho, Mu-Yeol, Lee, Eun-Song, Jung, Hoi In, Kim, Baek-Il
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467919/
https://www.ncbi.nlm.nih.gov/pubmed/32879370
http://dx.doi.org/10.1038/s41598-020-71363-6
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author Kim, Hyo-Jung
Cho, Mu-Yeol
Lee, Eun-Song
Jung, Hoi In
Kim, Baek-Il
author_facet Kim, Hyo-Jung
Cho, Mu-Yeol
Lee, Eun-Song
Jung, Hoi In
Kim, Baek-Il
author_sort Kim, Hyo-Jung
collection PubMed
description This study evaluated the antibacterial effects of short-time exposure of surface pre-reacted glass-ionomer (S-PRG) eluate on oral microcosm biofilm. Biofilms were treated with an S-PRG eluate at different concentrations (25%, 50%, and 100%), distilled water (DW), and 0.1% chlorhexidine (CHX) twice a day for 5 min repeatedly. After 7 days, the total and aciduric bacterial counts and biofilm dry weights were measured. An image analysis program calculated the red/green (R/G) ratios in the biofilm autofluorescence images. Microscopic analyses quantified the biofilm thickness and live/dead cell ratio and determined morphological changes in the biofilm. Bacterial counts and dry weights were not significantly different in the DW group for all S-PRG eluate concentrations. An increasing trend in the R/G ratio for 7 days biofilm treatment was observed for the S-PRG eluate and the DW groups. Furthermore, the live/dead cell ratios in the biofilm and the biofilm thickness of the S-PRG eluate groups were similar to those of the DW group. The bacteria morphology inside the biofilm changed only in the CHX group. Short-time S-PRG eluate treatment showed no significant antibacterial and antibiofilm effects. These results indicated that limited biofilm formation inhibition can be obtained by using only the S-PRG eluate.
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spelling pubmed-74679192020-09-03 Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm Kim, Hyo-Jung Cho, Mu-Yeol Lee, Eun-Song Jung, Hoi In Kim, Baek-Il Sci Rep Article This study evaluated the antibacterial effects of short-time exposure of surface pre-reacted glass-ionomer (S-PRG) eluate on oral microcosm biofilm. Biofilms were treated with an S-PRG eluate at different concentrations (25%, 50%, and 100%), distilled water (DW), and 0.1% chlorhexidine (CHX) twice a day for 5 min repeatedly. After 7 days, the total and aciduric bacterial counts and biofilm dry weights were measured. An image analysis program calculated the red/green (R/G) ratios in the biofilm autofluorescence images. Microscopic analyses quantified the biofilm thickness and live/dead cell ratio and determined morphological changes in the biofilm. Bacterial counts and dry weights were not significantly different in the DW group for all S-PRG eluate concentrations. An increasing trend in the R/G ratio for 7 days biofilm treatment was observed for the S-PRG eluate and the DW groups. Furthermore, the live/dead cell ratios in the biofilm and the biofilm thickness of the S-PRG eluate groups were similar to those of the DW group. The bacteria morphology inside the biofilm changed only in the CHX group. Short-time S-PRG eluate treatment showed no significant antibacterial and antibiofilm effects. These results indicated that limited biofilm formation inhibition can be obtained by using only the S-PRG eluate. Nature Publishing Group UK 2020-09-02 /pmc/articles/PMC7467919/ /pubmed/32879370 http://dx.doi.org/10.1038/s41598-020-71363-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Kim, Hyo-Jung
Cho, Mu-Yeol
Lee, Eun-Song
Jung, Hoi In
Kim, Baek-Il
Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title_full Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title_fullStr Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title_full_unstemmed Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title_short Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
title_sort effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467919/
https://www.ncbi.nlm.nih.gov/pubmed/32879370
http://dx.doi.org/10.1038/s41598-020-71363-6
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