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Ocular conjunctival inoculation of SARS-CoV-2 can cause mild COVID-19 in rhesus macaques

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the respiratory route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1 × 10(6) TCID(50) of SARS-CoV-2 conjunctivally (CJ), int...

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Detalles Bibliográficos
Autores principales: Deng, Wei, Bao, Linlin, Gao, Hong, Xiang, Zhiguang, Qu, Yajin, Song, Zhiqi, Gong, Shuran, Liu, Jiayi, Liu, Jiangning, Yu, Pin, Qi, Feifei, Xu, Yanfeng, Li, Fengli, Xiao, Chong, Lv, Qi, Xue, Jing, Wei, Qiang, Liu, Mingya, Wang, Guanpeng, Wang, Shunyi, Yu, Haisheng, Chen, Ting, Liu, Xing, Zhao, Wenjie, Han, Yunlin, Qin, Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467924/
https://www.ncbi.nlm.nih.gov/pubmed/32879306
http://dx.doi.org/10.1038/s41467-020-18149-6
Descripción
Sumario:Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is highly transmitted through the respiratory route, but potential extra-respiratory routes of SARS-CoV-2 transmission remain uncertain. Here we inoculated five rhesus macaques with 1 × 10(6) TCID(50) of SARS-CoV-2 conjunctivally (CJ), intratracheally (IT), and intragastrically (IG). Nasal and throat swabs collected from CJ and IT had detectable viral RNA at 1–7 days post-inoculation (dpi). Viral RNA was detected in anal swabs from only the IT group at 1–7 dpi. Viral RNA was undetectable in tested swabs and tissues after intragastric inoculation. The CJ infected animal had a higher viral load in the nasolacrimal system than the IT infected animal but also showed mild interstitial pneumonia, suggesting distinct virus distributions. This study shows that infection via the conjunctival route is possible in non-human primates; further studies are necessary to compare the relative risk and pathogenesis of infection through these different routes in more detail.