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KIR and HLA-C genes in male infertility

PURPOSE: Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammator...

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Autores principales: Wilczyńska, Karolina, Radwan, Paweł, Krasiński, Rafał, Radwan, Michał, Wilczyński, Jacek R., Malinowski, Andrzej, Barcz, Ewa, Nowak, Izabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467998/
https://www.ncbi.nlm.nih.gov/pubmed/32436047
http://dx.doi.org/10.1007/s10815-020-01814-6
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author Wilczyńska, Karolina
Radwan, Paweł
Krasiński, Rafał
Radwan, Michał
Wilczyński, Jacek R.
Malinowski, Andrzej
Barcz, Ewa
Nowak, Izabela
author_facet Wilczyńska, Karolina
Radwan, Paweł
Krasiński, Rafał
Radwan, Michał
Wilczyński, Jacek R.
Malinowski, Andrzej
Barcz, Ewa
Nowak, Izabela
author_sort Wilczyńska, Karolina
collection PubMed
description PURPOSE: Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis. METHOD: We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods. RESULTS: We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/p(corr.) = 0.0015/0.035, OR = 1.61), KIR2DL5 gr.2 (p/p(corr.) = 0.0023/0.05, OR = 1.64), KIR2DS2 (p/p(corr.) = 0.0019/0.044, OR = 1.59), and KIR2DS3 (p/p(corr.) = 0.0016/0.037, OR = 1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/p(corr.) = 0.0076/0.03, OR = 0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/p(corr.) = 0.0076/0.03, OR = 1.50). We also observed a limited association in KIR-HLA-C combinations. CONCLUSION: Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-020-01814-6) contains supplementary material, which is available to authorized users.
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spelling pubmed-74679982020-09-15 KIR and HLA-C genes in male infertility Wilczyńska, Karolina Radwan, Paweł Krasiński, Rafał Radwan, Michał Wilczyński, Jacek R. Malinowski, Andrzej Barcz, Ewa Nowak, Izabela J Assist Reprod Genet Genetics PURPOSE: Approximately 50% of men reporting to clinics for assisted reproduction have abnormal sperm parameters; we therefore considered whether they differ from fertile males in terms of the frequency of KIR and HLA-C genes, suggesting the involvement of NK cells and some T cells in the inflammatory reaction that can occur in the testes, vas deferens, or epididymis. METHOD: We tested a total of 1064 men: 445 of them were patients who, together with their female partners, participated in in vitro fertilization (IVF), 298 men whose female partners suffered from recurrent spontaneous abortion. Three hundred twenty-one fertile men constituted the control group. KIRs were genotyped using KIR Ready Gene kits and HLA-C by PCR-SSP methods. RESULTS: We found differences in KIR gene frequencies between men who became fathers via natural conception and men who participated in in vitro fertilization for KIR2DL2 (p/p(corr.) = 0.0015/0.035, OR = 1.61), KIR2DL5 gr.2 (p/p(corr.) = 0.0023/0.05, OR = 1.64), KIR2DS2 (p/p(corr.) = 0.0019/0.044, OR = 1.59), and KIR2DS3 (p/p(corr.) = 0.0016/0.037, OR = 1.67). KIRs in Cen AA region were significantly overrepresented in fertile males than in IVF males (p/p(corr.) = 0.0076/0.03, OR = 0.67), whereas Cen AB + Cen BB frequency was higher in IVF males than in fertile males (p/p(corr.) = 0.0076/0.03, OR = 1.50). We also observed a limited association in KIR-HLA-C combinations. CONCLUSION: Fertile men differ in profile of KIR genes and KIR-HLA-C combinations from men participating in IVF. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s10815-020-01814-6) contains supplementary material, which is available to authorized users. Springer US 2020-05-20 2020-08 /pmc/articles/PMC7467998/ /pubmed/32436047 http://dx.doi.org/10.1007/s10815-020-01814-6 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics
Wilczyńska, Karolina
Radwan, Paweł
Krasiński, Rafał
Radwan, Michał
Wilczyński, Jacek R.
Malinowski, Andrzej
Barcz, Ewa
Nowak, Izabela
KIR and HLA-C genes in male infertility
title KIR and HLA-C genes in male infertility
title_full KIR and HLA-C genes in male infertility
title_fullStr KIR and HLA-C genes in male infertility
title_full_unstemmed KIR and HLA-C genes in male infertility
title_short KIR and HLA-C genes in male infertility
title_sort kir and hla-c genes in male infertility
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7467998/
https://www.ncbi.nlm.nih.gov/pubmed/32436047
http://dx.doi.org/10.1007/s10815-020-01814-6
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