miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer

The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared wi...

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Autores principales: Wang, Hui, Hu, Hui, Luo, Zhenzhao, Liu, Shuiyi, Wu, Wangze, Zhu, Man, Wang, Jing, Liu, Yingle, Lu, Zhongxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Cancer
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468098/
https://www.ncbi.nlm.nih.gov/pubmed/32816024
http://dx.doi.org/10.1042/BSR20200796
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author Wang, Hui
Hu, Hui
Luo, Zhenzhao
Liu, Shuiyi
Wu, Wangze
Zhu, Man
Wang, Jing
Liu, Yingle
Lu, Zhongxin
author_facet Wang, Hui
Hu, Hui
Luo, Zhenzhao
Liu, Shuiyi
Wu, Wangze
Zhu, Man
Wang, Jing
Liu, Yingle
Lu, Zhongxin
author_sort Wang, Hui
collection PubMed
description The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells.
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spelling pubmed-74680982020-09-11 miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer Wang, Hui Hu, Hui Luo, Zhenzhao Liu, Shuiyi Wu, Wangze Zhu, Man Wang, Jing Liu, Yingle Lu, Zhongxin Biosci Rep Cancer The abnormal expression of HPV16 E6/E7 activates oncogenes and/or inactivates tumor suppressor genes, resulting in the selective growth and malignant transformation of cancer cells. miR-4454 was selected by sequencing due to its abnormal high expression in HPV16 E6/E7 positive CaSki cell compared with HPV16 E6/E7 negative C33A cell. Overexpression of miR-4454 enhances cervical cancer cell invasion and migration. ABHD2 and NUDT21 are identified as a target gene of miR-4454.The effects of ABHD2 and NUDT21 on migration and invasion of CaSki and C33A cells were determined. The dual luciferase and RT-qPCR assays confirmed that miR-4454 might regulate its targets ABHD2 and NUDT21 to promote the proliferation, invasion and migration, whereas, inhibit the apoptosis in CaSki and C33A cells. Portland Press Ltd. 2020-09-02 /pmc/articles/PMC7468098/ /pubmed/32816024 http://dx.doi.org/10.1042/BSR20200796 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Cancer
Wang, Hui
Hu, Hui
Luo, Zhenzhao
Liu, Shuiyi
Wu, Wangze
Zhu, Man
Wang, Jing
Liu, Yingle
Lu, Zhongxin
miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title_full miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title_fullStr miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title_full_unstemmed miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title_short miR-4454 up-regulated by HPV16 E6/E7 promotes invasion and migration by targeting ABHD2/NUDT21 in cervical cancer
title_sort mir-4454 up-regulated by hpv16 e6/e7 promotes invasion and migration by targeting abhd2/nudt21 in cervical cancer
topic Cancer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468098/
https://www.ncbi.nlm.nih.gov/pubmed/32816024
http://dx.doi.org/10.1042/BSR20200796
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