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Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon

Snakebites are considered a major public health problem worldwide. In the Amazon region of Brazil, the snake Bothrops atrox (B. atrox) is responsible for 90% of the bites. These bites may cause local and systemic signs from acute inflammatory reaction and hemostatic changes, and present common hemor...

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Autores principales: Wellmann, Irmgardt Alicia María, Ibiapina, Hiochelson Najibe Santos, Sachett, Jacqueline Almeida Gonçalves, Sartim, Marco Aurélio, Silva, Iran Mendonça, Oliveira, Sâmella Silva, Tarragô, Andréa Monteiro, Moura-da-Silva, Ana Maria, Lacerda, Marcus Vinícius Guimarães, Ferreira, Luiz Carlos de Lima, Malheiro, Adriana, Monteiro, Wuelton Marcelo, Costa, Allyson Guimarães
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468254/
https://www.ncbi.nlm.nih.gov/pubmed/32973773
http://dx.doi.org/10.3389/fimmu.2020.01874
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author Wellmann, Irmgardt Alicia María
Ibiapina, Hiochelson Najibe Santos
Sachett, Jacqueline Almeida Gonçalves
Sartim, Marco Aurélio
Silva, Iran Mendonça
Oliveira, Sâmella Silva
Tarragô, Andréa Monteiro
Moura-da-Silva, Ana Maria
Lacerda, Marcus Vinícius Guimarães
Ferreira, Luiz Carlos de Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
author_facet Wellmann, Irmgardt Alicia María
Ibiapina, Hiochelson Najibe Santos
Sachett, Jacqueline Almeida Gonçalves
Sartim, Marco Aurélio
Silva, Iran Mendonça
Oliveira, Sâmella Silva
Tarragô, Andréa Monteiro
Moura-da-Silva, Ana Maria
Lacerda, Marcus Vinícius Guimarães
Ferreira, Luiz Carlos de Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
author_sort Wellmann, Irmgardt Alicia María
collection PubMed
description Snakebites are considered a major public health problem worldwide. In the Amazon region of Brazil, the snake Bothrops atrox (B. atrox) is responsible for 90% of the bites. These bites may cause local and systemic signs from acute inflammatory reaction and hemostatic changes, and present common hemorrhagic disorders. These alterations occur due the action of hemostatically active and immunogenic toxins which are capable of triggering a wide range of hemostatic and inflammatory events. However, the crosstalk between coagulation disorders and inflammatory reaction still has gaps in snakebites. Thus, the goal of this study was to describe the relationship between the consumption of fibrinogen and the profile of inflammatory molecules (chemokines and cytokines) in evenomations by B. atrox snakebites. A prospective study was carried out with individuals who had suffered B. atrox snakebites and presented different levels of fibrinogen consumption (normal fibrinogen [NF] and hypofibrinogenemia [HF]). Seventeen patients with NF and 55 patients with HF were eligible for the study, in addition to 50 healthy controls (CG). The molecules CXCL-8, CCL-5, CXCL-9, CCL-2, CXCL-10, IL-6, TNF, IL-2, IL-10, IFN-γ, IL-4, and IL-17A were quantified in plasma using the CBA technique at three different times (pre-antivenom therapy [T0], 24 h [T1], and 48 h [T2] after antivenom therapy). The profile of the circulating inflammatory response is different between the groups studied, with HF patients having higher concentrations of CCL-5 and lower IFN-γ. In addition, antivenom therapy seems to have a positive effect, leading to a profile of circulating inflammatory response similar in quantification of T1 and T2 on both groups. Furthermore, these results suggest that a number of interactions of CXCL-8, CXCL-9, CCL-2, IL-6, and IFN-γ in HF patients are directly affected by fibrinogen levels, which may be related to the inflammatory response and coagulation mutual relationship induced by B. atrox venom. The present study is the first report on inflammation-coagulation crosstalk involving snakebite patients and supports the better understanding of envenomation's pathophysiology mechanisms and guides in the search for novel biomarkers and prospective therapies.
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spelling pubmed-74682542020-09-23 Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon Wellmann, Irmgardt Alicia María Ibiapina, Hiochelson Najibe Santos Sachett, Jacqueline Almeida Gonçalves Sartim, Marco Aurélio Silva, Iran Mendonça Oliveira, Sâmella Silva Tarragô, Andréa Monteiro Moura-da-Silva, Ana Maria Lacerda, Marcus Vinícius Guimarães Ferreira, Luiz Carlos de Lima Malheiro, Adriana Monteiro, Wuelton Marcelo Costa, Allyson Guimarães Front Immunol Immunology Snakebites are considered a major public health problem worldwide. In the Amazon region of Brazil, the snake Bothrops atrox (B. atrox) is responsible for 90% of the bites. These bites may cause local and systemic signs from acute inflammatory reaction and hemostatic changes, and present common hemorrhagic disorders. These alterations occur due the action of hemostatically active and immunogenic toxins which are capable of triggering a wide range of hemostatic and inflammatory events. However, the crosstalk between coagulation disorders and inflammatory reaction still has gaps in snakebites. Thus, the goal of this study was to describe the relationship between the consumption of fibrinogen and the profile of inflammatory molecules (chemokines and cytokines) in evenomations by B. atrox snakebites. A prospective study was carried out with individuals who had suffered B. atrox snakebites and presented different levels of fibrinogen consumption (normal fibrinogen [NF] and hypofibrinogenemia [HF]). Seventeen patients with NF and 55 patients with HF were eligible for the study, in addition to 50 healthy controls (CG). The molecules CXCL-8, CCL-5, CXCL-9, CCL-2, CXCL-10, IL-6, TNF, IL-2, IL-10, IFN-γ, IL-4, and IL-17A were quantified in plasma using the CBA technique at three different times (pre-antivenom therapy [T0], 24 h [T1], and 48 h [T2] after antivenom therapy). The profile of the circulating inflammatory response is different between the groups studied, with HF patients having higher concentrations of CCL-5 and lower IFN-γ. In addition, antivenom therapy seems to have a positive effect, leading to a profile of circulating inflammatory response similar in quantification of T1 and T2 on both groups. Furthermore, these results suggest that a number of interactions of CXCL-8, CXCL-9, CCL-2, IL-6, and IFN-γ in HF patients are directly affected by fibrinogen levels, which may be related to the inflammatory response and coagulation mutual relationship induced by B. atrox venom. The present study is the first report on inflammation-coagulation crosstalk involving snakebite patients and supports the better understanding of envenomation's pathophysiology mechanisms and guides in the search for novel biomarkers and prospective therapies. Frontiers Media S.A. 2020-08-18 /pmc/articles/PMC7468254/ /pubmed/32973773 http://dx.doi.org/10.3389/fimmu.2020.01874 Text en Copyright © 2020 Wellmann, Ibiapina, Sachett, Sartim, Silva, Oliveira, Tarragô, Moura-da-Silva, Lacerda, Ferreira, Malheiro, Monteiro and Costa. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Wellmann, Irmgardt Alicia María
Ibiapina, Hiochelson Najibe Santos
Sachett, Jacqueline Almeida Gonçalves
Sartim, Marco Aurélio
Silva, Iran Mendonça
Oliveira, Sâmella Silva
Tarragô, Andréa Monteiro
Moura-da-Silva, Ana Maria
Lacerda, Marcus Vinícius Guimarães
Ferreira, Luiz Carlos de Lima
Malheiro, Adriana
Monteiro, Wuelton Marcelo
Costa, Allyson Guimarães
Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title_full Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title_fullStr Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title_full_unstemmed Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title_short Correlating Fibrinogen Consumption and Profiles of Inflammatory Molecules in Human Envenomation's by Bothrops atrox in the Brazilian Amazon
title_sort correlating fibrinogen consumption and profiles of inflammatory molecules in human envenomation's by bothrops atrox in the brazilian amazon
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468254/
https://www.ncbi.nlm.nih.gov/pubmed/32973773
http://dx.doi.org/10.3389/fimmu.2020.01874
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