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Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut
The expansion of Enterobacteriaceae, such as E. coli is a main characteristic of gut inflammation and is related to multiple human diseases. However, how to control these E. coli overgrowth is not well understood. Here, we demonstrate that gut complement factor D (CFD) plays an important role in eli...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468294/ https://www.ncbi.nlm.nih.gov/pubmed/32879431 http://dx.doi.org/10.1038/s42003-020-01219-2 |
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author | Qi, Houbao Wei, Jianmei Gao, Yunhuan Yang, Yazheng Li, Yuanyuan Zhu, Hua Su, Lei Su, Xiaomin Zhang, Yuan Yang, Rongcun |
author_facet | Qi, Houbao Wei, Jianmei Gao, Yunhuan Yang, Yazheng Li, Yuanyuan Zhu, Hua Su, Lei Su, Xiaomin Zhang, Yuan Yang, Rongcun |
author_sort | Qi, Houbao |
collection | PubMed |
description | The expansion of Enterobacteriaceae, such as E. coli is a main characteristic of gut inflammation and is related to multiple human diseases. However, how to control these E. coli overgrowth is not well understood. Here, we demonstrate that gut complement factor D (CFD) plays an important role in eliminating E. coli. Increased E. coli, which could stimulate inflammatory macrophages to induce colitis, were found in the gut of CFD deficient mice. We also showed that gut Reg4, which is expressed in gut epithelial cells, stimulated complement-mediated attack complexes to eliminate E. coli. Reg4 deficient mice also had increased E. coli. The dominant E. coli were isolated from colitis tissues of mice and found to be sensitive to both CFD- and Reg4-mediated attack complexes. Thus, gut Reg4- and CFD-mediated membrane attack complexes may maintain gut homeostasis by killing inflammatory E. coli. |
format | Online Article Text |
id | pubmed-7468294 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74682942020-09-16 Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut Qi, Houbao Wei, Jianmei Gao, Yunhuan Yang, Yazheng Li, Yuanyuan Zhu, Hua Su, Lei Su, Xiaomin Zhang, Yuan Yang, Rongcun Commun Biol Article The expansion of Enterobacteriaceae, such as E. coli is a main characteristic of gut inflammation and is related to multiple human diseases. However, how to control these E. coli overgrowth is not well understood. Here, we demonstrate that gut complement factor D (CFD) plays an important role in eliminating E. coli. Increased E. coli, which could stimulate inflammatory macrophages to induce colitis, were found in the gut of CFD deficient mice. We also showed that gut Reg4, which is expressed in gut epithelial cells, stimulated complement-mediated attack complexes to eliminate E. coli. Reg4 deficient mice also had increased E. coli. The dominant E. coli were isolated from colitis tissues of mice and found to be sensitive to both CFD- and Reg4-mediated attack complexes. Thus, gut Reg4- and CFD-mediated membrane attack complexes may maintain gut homeostasis by killing inflammatory E. coli. Nature Publishing Group UK 2020-09-02 /pmc/articles/PMC7468294/ /pubmed/32879431 http://dx.doi.org/10.1038/s42003-020-01219-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Qi, Houbao Wei, Jianmei Gao, Yunhuan Yang, Yazheng Li, Yuanyuan Zhu, Hua Su, Lei Su, Xiaomin Zhang, Yuan Yang, Rongcun Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title | Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title_full | Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title_fullStr | Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title_full_unstemmed | Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title_short | Reg4 and complement factor D prevent the overgrowth of E. coli in the mouse gut |
title_sort | reg4 and complement factor d prevent the overgrowth of e. coli in the mouse gut |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468294/ https://www.ncbi.nlm.nih.gov/pubmed/32879431 http://dx.doi.org/10.1038/s42003-020-01219-2 |
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