Rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA

Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capaci...

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Detalles Bibliográficos
Autores principales: Michel, Detlef, Danzer, Karin M., Groß, Rüdiger, Conzelmann, Carina, Müller, Janis A., Freischmidt, Axel, Weishaupt, Jochen H., Heller, Sandra, Münch, Jan, Michel, Manuela, Stamminger, Thomas, Kleger, Alexander, Otto, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2020
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468345/
https://www.ncbi.nlm.nih.gov/pubmed/32891677
http://dx.doi.org/10.1016/j.jviromet.2020.113965
Descripción
Sumario:Pandemic SARS-CoV-2 infection has rapidly developed into a socioeconomic and humanitarian catastrophe. Basic principles to prevent SARS-CoV-2 transmission are social distancing, face masks, contact tracing and early detection of SARS-CoV-2. To meet these requirements, virtually unlimited test capacities delivering results in a rapid and reliable manner are a prerequisite. Here, we provide and validate such a rapid, convenient and efficient kit-independent detection of SARS-CoV-2 RNA, termed COVID-quick-DET. This straightforward method operates with simple proteinase K treatment and repetitive heating steps with a sensitivity of 94.6% in head-to-head comparisons with kit-based isolation methods. This result is supported by data obtained from serially diluted SARS-CoV-2 virus stocks. Given its cost- and time-effective operation, COVID-quick-DET might be best suited for countries with general shortage or temporary acute scarcity of resources and equipment.

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