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Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)

Black dot is a blemish disease of potato tubers caused by the phytopathogenic fungus Colletotrichum coccodes. Qualitative resistance (monogenic) that leads to the hypersensitive response has not been reported against black dot, but commercial potato cultivars show different susceptibility levels to...

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Autores principales: Massana-Codina, Josep, Schnee, Sylvain, Allard, Pierre-Marie, Rutz, Adriano, Boccard, Julien, Michellod, Emilie, Cléroux, Marilyn, Schürch, Stéphanie, Gindro, Katia, Wolfender, Jean-Luc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468465/
https://www.ncbi.nlm.nih.gov/pubmed/32973846
http://dx.doi.org/10.3389/fpls.2020.01287
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author Massana-Codina, Josep
Schnee, Sylvain
Allard, Pierre-Marie
Rutz, Adriano
Boccard, Julien
Michellod, Emilie
Cléroux, Marilyn
Schürch, Stéphanie
Gindro, Katia
Wolfender, Jean-Luc
author_facet Massana-Codina, Josep
Schnee, Sylvain
Allard, Pierre-Marie
Rutz, Adriano
Boccard, Julien
Michellod, Emilie
Cléroux, Marilyn
Schürch, Stéphanie
Gindro, Katia
Wolfender, Jean-Luc
author_sort Massana-Codina, Josep
collection PubMed
description Black dot is a blemish disease of potato tubers caused by the phytopathogenic fungus Colletotrichum coccodes. Qualitative resistance (monogenic) that leads to the hypersensitive response has not been reported against black dot, but commercial potato cultivars show different susceptibility levels to the disease, indicating that quantitative resistance (polygenic) mechanisms against this pathogen exist. Cytological studies are essential to decipher pathogen colonization of the plant tissue, and untargeted metabolomics has been shown effective in highlighting resistance-related metabolites in quantitative resistance. In this study, we used five commercial potato cultivars with different susceptibility levels to black dot, and studied the structural and biochemical aspects that correlate with resistance to black dot using cytological and untargeted metabolomics methods. The cytological approach using semithin sections of potato tuber periderm revealed that C. coccodes colonizes the tuber periderm, but does not penetrate in cortical cells. Furthermore, skin thickness did not correlate with disease susceptibility, indicating that other factors influence quantitative resistance to black dot. Furthermore, suberin amounts did not correlate with black dot severity, and suberin composition was similar between the five potato cultivars studied. On the other hand, the untargeted metabolomics approach allowed highlighting biomarkers of infection, as well as constitutive and induced resistance-related metabolites. Hydroxycinnamic acids, hydroxycinnamic acid amides and steroidal saponins were found to be biomarkers of resistance under control conditions, while hydroxycoumarins were found to be specifically induced in the resistant cultivars. Notably, some of these biomarkers showed antifungal activity in vitro against C. coccodes. Altogether, our results show that quantitative resistance of potatoes to black dot involves structural and biochemical mechanisms, including the production of specialized metabolites with antifungal properties.
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spelling pubmed-74684652020-09-23 Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes) Massana-Codina, Josep Schnee, Sylvain Allard, Pierre-Marie Rutz, Adriano Boccard, Julien Michellod, Emilie Cléroux, Marilyn Schürch, Stéphanie Gindro, Katia Wolfender, Jean-Luc Front Plant Sci Plant Science Black dot is a blemish disease of potato tubers caused by the phytopathogenic fungus Colletotrichum coccodes. Qualitative resistance (monogenic) that leads to the hypersensitive response has not been reported against black dot, but commercial potato cultivars show different susceptibility levels to the disease, indicating that quantitative resistance (polygenic) mechanisms against this pathogen exist. Cytological studies are essential to decipher pathogen colonization of the plant tissue, and untargeted metabolomics has been shown effective in highlighting resistance-related metabolites in quantitative resistance. In this study, we used five commercial potato cultivars with different susceptibility levels to black dot, and studied the structural and biochemical aspects that correlate with resistance to black dot using cytological and untargeted metabolomics methods. The cytological approach using semithin sections of potato tuber periderm revealed that C. coccodes colonizes the tuber periderm, but does not penetrate in cortical cells. Furthermore, skin thickness did not correlate with disease susceptibility, indicating that other factors influence quantitative resistance to black dot. Furthermore, suberin amounts did not correlate with black dot severity, and suberin composition was similar between the five potato cultivars studied. On the other hand, the untargeted metabolomics approach allowed highlighting biomarkers of infection, as well as constitutive and induced resistance-related metabolites. Hydroxycinnamic acids, hydroxycinnamic acid amides and steroidal saponins were found to be biomarkers of resistance under control conditions, while hydroxycoumarins were found to be specifically induced in the resistant cultivars. Notably, some of these biomarkers showed antifungal activity in vitro against C. coccodes. Altogether, our results show that quantitative resistance of potatoes to black dot involves structural and biochemical mechanisms, including the production of specialized metabolites with antifungal properties. Frontiers Media S.A. 2020-08-20 /pmc/articles/PMC7468465/ /pubmed/32973846 http://dx.doi.org/10.3389/fpls.2020.01287 Text en Copyright © 2020 Massana-Codina, Schnee, Allard, Rutz, Boccard, Michellod, Cléroux, Schürch, Gindro and Wolfender http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Massana-Codina, Josep
Schnee, Sylvain
Allard, Pierre-Marie
Rutz, Adriano
Boccard, Julien
Michellod, Emilie
Cléroux, Marilyn
Schürch, Stéphanie
Gindro, Katia
Wolfender, Jean-Luc
Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title_full Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title_fullStr Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title_full_unstemmed Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title_short Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)
title_sort insights on the structural and metabolic resistance of potato (solanum tuberosum) cultivars to tuber black dot (colletotrichum coccodes)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468465/
https://www.ncbi.nlm.nih.gov/pubmed/32973846
http://dx.doi.org/10.3389/fpls.2020.01287
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