Cargando…
Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits
This study deals with α-glucosidase and β-secretase inhibitory screening of extract/fractions and isolated daturaolone (1), namely, 3-oxo-6-β-hydroxy-β-amyrin (daturaolone) from chloroform fraction of Datura metel L. Among entire fractions, the chloroform soluble fraction showed excellent activity a...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468596/ https://www.ncbi.nlm.nih.gov/pubmed/32908922 http://dx.doi.org/10.1155/2020/8530165 |
_version_ | 1783578251802705920 |
---|---|
author | Bawazeer, Saud Rauf, Abdur Bawazeer, Sami |
author_facet | Bawazeer, Saud Rauf, Abdur Bawazeer, Sami |
author_sort | Bawazeer, Saud |
collection | PubMed |
description | This study deals with α-glucosidase and β-secretase inhibitory screening of extract/fractions and isolated daturaolone (1), namely, 3-oxo-6-β-hydroxy-β-amyrin (daturaolone) from chloroform fraction of Datura metel L. Among entire fractions, the chloroform soluble fraction showed excellent activity against α-glucosidase with % inhibition 90.8 with IC(50)160.2 ± 1.85 μg and daturaolone (1) with 98.7% inhibition with IC(50)840.4 ± 1.74 μM, respectively. Similarly, extract and daturaolone (1) also exhibited significant activity against the β-secretase enzyme (BACE1) with % activities 88.27 and 95.19 and with IC(50) values 304.21 ± 2.98 μg and 260.70 ± 1.87 μM, respectively, as compared to the standard inhibitor (Ans670, Sta671, Val672)-amyloid-β/A4 precursor protein 770 fragments 662-675) with % activity 94.21 and IC(50) value 289.24 ± 1.60 μM. This finding encourages and opens a new window for further detail phytochemical investigation on D. metel in order to isolate novel compounds with promising enzyme inhibitory potential. |
format | Online Article Text |
id | pubmed-7468596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-74685962020-09-08 Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits Bawazeer, Saud Rauf, Abdur Bawazeer, Sami Biomed Res Int Research Article This study deals with α-glucosidase and β-secretase inhibitory screening of extract/fractions and isolated daturaolone (1), namely, 3-oxo-6-β-hydroxy-β-amyrin (daturaolone) from chloroform fraction of Datura metel L. Among entire fractions, the chloroform soluble fraction showed excellent activity against α-glucosidase with % inhibition 90.8 with IC(50)160.2 ± 1.85 μg and daturaolone (1) with 98.7% inhibition with IC(50)840.4 ± 1.74 μM, respectively. Similarly, extract and daturaolone (1) also exhibited significant activity against the β-secretase enzyme (BACE1) with % activities 88.27 and 95.19 and with IC(50) values 304.21 ± 2.98 μg and 260.70 ± 1.87 μM, respectively, as compared to the standard inhibitor (Ans670, Sta671, Val672)-amyloid-β/A4 precursor protein 770 fragments 662-675) with % activity 94.21 and IC(50) value 289.24 ± 1.60 μM. This finding encourages and opens a new window for further detail phytochemical investigation on D. metel in order to isolate novel compounds with promising enzyme inhibitory potential. Hindawi 2020-08-25 /pmc/articles/PMC7468596/ /pubmed/32908922 http://dx.doi.org/10.1155/2020/8530165 Text en Copyright © 2020 Saud Bawazeer et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Bawazeer, Saud Rauf, Abdur Bawazeer, Sami Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title | Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title_full | Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title_fullStr | Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title_full_unstemmed | Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title_short | Potent In Vitro α-Glucosidase and β-Secretase Inhibition of Amyrin-Type Triterpenoid Isolated from Datura metel Linnaeus (Angel's Trumpet) Fruits |
title_sort | potent in vitro α-glucosidase and β-secretase inhibition of amyrin-type triterpenoid isolated from datura metel linnaeus (angel's trumpet) fruits |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468596/ https://www.ncbi.nlm.nih.gov/pubmed/32908922 http://dx.doi.org/10.1155/2020/8530165 |
work_keys_str_mv | AT bawazeersaud potentinvitroaglucosidaseandbsecretaseinhibitionofamyrintypetriterpenoidisolatedfromdaturametellinnaeusangelstrumpetfruits AT raufabdur potentinvitroaglucosidaseandbsecretaseinhibitionofamyrintypetriterpenoidisolatedfromdaturametellinnaeusangelstrumpetfruits AT bawazeersami potentinvitroaglucosidaseandbsecretaseinhibitionofamyrintypetriterpenoidisolatedfromdaturametellinnaeusangelstrumpetfruits |