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Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis

Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disea...

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Autores principales: She, Chunhui, Wang, Jing, Tang, Ning, Liu, Zhaoyang, Xu, Lishan, Liu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468604/
https://www.ncbi.nlm.nih.gov/pubmed/32908870
http://dx.doi.org/10.1155/2020/1754975
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author She, Chunhui
Wang, Jing
Tang, Ning
Liu, Zhaoyang
Xu, Lishan
Liu, Bin
author_facet She, Chunhui
Wang, Jing
Tang, Ning
Liu, Zhaoyang
Xu, Lishan
Liu, Bin
author_sort She, Chunhui
collection PubMed
description Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors. This study showed the effect of dendritic cells (DCs) activated by TCDD on naïve CD4(+) T cell differentiation in patients with PBC. CD14(+) mononuclear cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with PBC and healthy people by magnetic cell separation and introduced into DCs. Two days after stimulation by TCDD, DCs were cocultured with naïve CD4(+) T cells in a ratio of 1 : 2 for 3 days. Then, differentiation-related factors for naïve CD4(+) T cells were detected by real-time fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and flow cytometry. The results showed that TCDD-activated DCs could promote Th1 and Th17 differentiation in patients with PBC. Therefore, this study demonstrated TCDD as an AhR agonist in regulating naïve CD4(+) T cell differentiation in patients with PBC.
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spelling pubmed-74686042020-09-08 Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis She, Chunhui Wang, Jing Tang, Ning Liu, Zhaoyang Xu, Lishan Liu, Bin Biomed Res Int Research Article Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors. This study showed the effect of dendritic cells (DCs) activated by TCDD on naïve CD4(+) T cell differentiation in patients with PBC. CD14(+) mononuclear cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with PBC and healthy people by magnetic cell separation and introduced into DCs. Two days after stimulation by TCDD, DCs were cocultured with naïve CD4(+) T cells in a ratio of 1 : 2 for 3 days. Then, differentiation-related factors for naïve CD4(+) T cells were detected by real-time fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and flow cytometry. The results showed that TCDD-activated DCs could promote Th1 and Th17 differentiation in patients with PBC. Therefore, this study demonstrated TCDD as an AhR agonist in regulating naïve CD4(+) T cell differentiation in patients with PBC. Hindawi 2020-08-24 /pmc/articles/PMC7468604/ /pubmed/32908870 http://dx.doi.org/10.1155/2020/1754975 Text en Copyright © 2020 Chunhui She et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
She, Chunhui
Wang, Jing
Tang, Ning
Liu, Zhaoyang
Xu, Lishan
Liu, Bin
Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title_full Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title_fullStr Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title_full_unstemmed Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title_short Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
title_sort effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on t cell differentiation in primary biliary cholangitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468604/
https://www.ncbi.nlm.nih.gov/pubmed/32908870
http://dx.doi.org/10.1155/2020/1754975
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