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Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis
Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disea...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468604/ https://www.ncbi.nlm.nih.gov/pubmed/32908870 http://dx.doi.org/10.1155/2020/1754975 |
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author | She, Chunhui Wang, Jing Tang, Ning Liu, Zhaoyang Xu, Lishan Liu, Bin |
author_facet | She, Chunhui Wang, Jing Tang, Ning Liu, Zhaoyang Xu, Lishan Liu, Bin |
author_sort | She, Chunhui |
collection | PubMed |
description | Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors. This study showed the effect of dendritic cells (DCs) activated by TCDD on naïve CD4(+) T cell differentiation in patients with PBC. CD14(+) mononuclear cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with PBC and healthy people by magnetic cell separation and introduced into DCs. Two days after stimulation by TCDD, DCs were cocultured with naïve CD4(+) T cells in a ratio of 1 : 2 for 3 days. Then, differentiation-related factors for naïve CD4(+) T cells were detected by real-time fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and flow cytometry. The results showed that TCDD-activated DCs could promote Th1 and Th17 differentiation in patients with PBC. Therefore, this study demonstrated TCDD as an AhR agonist in regulating naïve CD4(+) T cell differentiation in patients with PBC. |
format | Online Article Text |
id | pubmed-7468604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-74686042020-09-08 Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis She, Chunhui Wang, Jing Tang, Ning Liu, Zhaoyang Xu, Lishan Liu, Bin Biomed Res Int Research Article Exposure to dioxins, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), is reported to affect the autoimmune system and increase the risk of autoimmune disease. Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors. This study showed the effect of dendritic cells (DCs) activated by TCDD on naïve CD4(+) T cell differentiation in patients with PBC. CD14(+) mononuclear cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with PBC and healthy people by magnetic cell separation and introduced into DCs. Two days after stimulation by TCDD, DCs were cocultured with naïve CD4(+) T cells in a ratio of 1 : 2 for 3 days. Then, differentiation-related factors for naïve CD4(+) T cells were detected by real-time fluorescence quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and flow cytometry. The results showed that TCDD-activated DCs could promote Th1 and Th17 differentiation in patients with PBC. Therefore, this study demonstrated TCDD as an AhR agonist in regulating naïve CD4(+) T cell differentiation in patients with PBC. Hindawi 2020-08-24 /pmc/articles/PMC7468604/ /pubmed/32908870 http://dx.doi.org/10.1155/2020/1754975 Text en Copyright © 2020 Chunhui She et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article She, Chunhui Wang, Jing Tang, Ning Liu, Zhaoyang Xu, Lishan Liu, Bin Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title | Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title_full | Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title_fullStr | Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title_full_unstemmed | Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title_short | Effects of 2,3,7,8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis |
title_sort | effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on t cell differentiation in primary biliary cholangitis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468604/ https://www.ncbi.nlm.nih.gov/pubmed/32908870 http://dx.doi.org/10.1155/2020/1754975 |
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