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The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice

Chronic isoleucine supplementation prevents diet-induced weight gain in rodents. Acute-isoleucine administration improves glucose tolerance in rodents and reduces postprandial glucose levels in humans. However, the effect of chronic-isoleucine supplementation on body weight and glucose tolerance in...

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Autores principales: O’Rielly, Rebecca, Li, Hui, Lim, See Meng, Yazbeck, Roger, Kritas, Stamatiki, Ullrich, Sina S., Feinle-Bisset, Christine, Heilbronn, Leonie, Page, Amanda J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468706/
https://www.ncbi.nlm.nih.gov/pubmed/32823899
http://dx.doi.org/10.3390/nu12082446
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author O’Rielly, Rebecca
Li, Hui
Lim, See Meng
Yazbeck, Roger
Kritas, Stamatiki
Ullrich, Sina S.
Feinle-Bisset, Christine
Heilbronn, Leonie
Page, Amanda J.
author_facet O’Rielly, Rebecca
Li, Hui
Lim, See Meng
Yazbeck, Roger
Kritas, Stamatiki
Ullrich, Sina S.
Feinle-Bisset, Christine
Heilbronn, Leonie
Page, Amanda J.
author_sort O’Rielly, Rebecca
collection PubMed
description Chronic isoleucine supplementation prevents diet-induced weight gain in rodents. Acute-isoleucine administration improves glucose tolerance in rodents and reduces postprandial glucose levels in humans. However, the effect of chronic-isoleucine supplementation on body weight and glucose tolerance in obesity is unknown. This study aimed to investigate the impact of chronic isoleucine on body weight gain and glucose tolerance in lean and high-fat-diet (HFD) induced-obese mice. Male C57BL/6-mice, fed a standard-laboratory-diet (SLD) or HFD for 12 weeks, were randomly allocated to: (1) Control: Drinking water; (2) Acute: Drinking water with a gavage of isoleucine (300 mg/kg) prior to the oral-glucose-tolerance-test (OGTT) or gastric-emptying-breath-test (GEBT); (3) Chronic: Drinking water with 1.5% isoleucine, for a further six weeks. At 16 weeks, an OGTT and GEBT was performed and at 17 weeks metabolic monitoring. In SLD- and HFD-mice, there was no difference in body weight, fat mass, and plasma lipid profiles between isoleucine treatment groups. Acute-isoleucine did not improve glucose tolerance in SLD- or HFD-mice. Chronic-isoleucine impaired glucose tolerance in SLD-mice. There was no difference in gastric emptying between any groups. Chronic-isoleucine did not alter energy intake, energy expenditure, or respiratory quotient in SLD- or HFD-mice. In conclusion, chronic isoleucine supplementation may not be an effective treatment for obesity or glucose intolerance.
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spelling pubmed-74687062020-09-04 The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice O’Rielly, Rebecca Li, Hui Lim, See Meng Yazbeck, Roger Kritas, Stamatiki Ullrich, Sina S. Feinle-Bisset, Christine Heilbronn, Leonie Page, Amanda J. Nutrients Article Chronic isoleucine supplementation prevents diet-induced weight gain in rodents. Acute-isoleucine administration improves glucose tolerance in rodents and reduces postprandial glucose levels in humans. However, the effect of chronic-isoleucine supplementation on body weight and glucose tolerance in obesity is unknown. This study aimed to investigate the impact of chronic isoleucine on body weight gain and glucose tolerance in lean and high-fat-diet (HFD) induced-obese mice. Male C57BL/6-mice, fed a standard-laboratory-diet (SLD) or HFD for 12 weeks, were randomly allocated to: (1) Control: Drinking water; (2) Acute: Drinking water with a gavage of isoleucine (300 mg/kg) prior to the oral-glucose-tolerance-test (OGTT) or gastric-emptying-breath-test (GEBT); (3) Chronic: Drinking water with 1.5% isoleucine, for a further six weeks. At 16 weeks, an OGTT and GEBT was performed and at 17 weeks metabolic monitoring. In SLD- and HFD-mice, there was no difference in body weight, fat mass, and plasma lipid profiles between isoleucine treatment groups. Acute-isoleucine did not improve glucose tolerance in SLD- or HFD-mice. Chronic-isoleucine impaired glucose tolerance in SLD-mice. There was no difference in gastric emptying between any groups. Chronic-isoleucine did not alter energy intake, energy expenditure, or respiratory quotient in SLD- or HFD-mice. In conclusion, chronic isoleucine supplementation may not be an effective treatment for obesity or glucose intolerance. MDPI 2020-08-14 /pmc/articles/PMC7468706/ /pubmed/32823899 http://dx.doi.org/10.3390/nu12082446 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
O’Rielly, Rebecca
Li, Hui
Lim, See Meng
Yazbeck, Roger
Kritas, Stamatiki
Ullrich, Sina S.
Feinle-Bisset, Christine
Heilbronn, Leonie
Page, Amanda J.
The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title_full The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title_fullStr The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title_full_unstemmed The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title_short The Effect of Isoleucine Supplementation on Body Weight Gain and Blood Glucose Response in Lean and Obese Mice
title_sort effect of isoleucine supplementation on body weight gain and blood glucose response in lean and obese mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468706/
https://www.ncbi.nlm.nih.gov/pubmed/32823899
http://dx.doi.org/10.3390/nu12082446
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