Cargando…

Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation

Previously, we have shown that oral administration of yeast derived β-1,3/1,6-d-glucan enhances immune regulation and alters the composition of the gut microbiota. However, it is not known if other structurally distinct β-glucans have similar properties. Here, using C57BL/6 mice, we show the potenti...

Descripción completa

Detalles Bibliográficos
Autores principales: Taylor, Harrison B., Gudi, Radhika, Brown, Robert, Vasu, Chenthamarakshan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468787/
https://www.ncbi.nlm.nih.gov/pubmed/32717991
http://dx.doi.org/10.3390/nu12082193
_version_ 1783578292976091136
author Taylor, Harrison B.
Gudi, Radhika
Brown, Robert
Vasu, Chenthamarakshan
author_facet Taylor, Harrison B.
Gudi, Radhika
Brown, Robert
Vasu, Chenthamarakshan
author_sort Taylor, Harrison B.
collection PubMed
description Previously, we have shown that oral administration of yeast derived β-1,3/1,6-d-glucan enhances immune regulation and alters the composition of the gut microbiota. However, it is not known if other structurally distinct β-glucans have similar properties. Here, using C57BL/6 mice, we show the potential of a microalgae derived β-1,3-d-glucan, paramylon (PM), in shaping the gut microbiota and modulating the susceptibility to colitis. The community structure within the gut microbiota showed progressive changes including selective enrichment of specific communities and lowered community richness and diversity during prolonged oral treatment with PM. Compared to control mice, the gut microbiota of PM-treated mice had significantly higher abundance of Verrucomicrobia and lower abundance of Firmicutes. Specific taxa that were significantly more abundant in PM-treated mice include Akkermansia muciniphila and several Bacteroides members. Predictive functional analysis revealed overrepresentation of carbohydrate metabolism function in the fecal microbiota of PM recipients compared to controls, and this function was linked to Bacteroides spp. Prolonged pretreatment with PM not only diminished susceptibility to dextran sulfate sodium induced colitis severity, but also caused enhanced immune regulation. Overall, this study demonstrates the prebiotic properties of PM and the potential benefits of its prolonged oral consumption to gut health.
format Online
Article
Text
id pubmed-7468787
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74687872020-09-04 Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation Taylor, Harrison B. Gudi, Radhika Brown, Robert Vasu, Chenthamarakshan Nutrients Article Previously, we have shown that oral administration of yeast derived β-1,3/1,6-d-glucan enhances immune regulation and alters the composition of the gut microbiota. However, it is not known if other structurally distinct β-glucans have similar properties. Here, using C57BL/6 mice, we show the potential of a microalgae derived β-1,3-d-glucan, paramylon (PM), in shaping the gut microbiota and modulating the susceptibility to colitis. The community structure within the gut microbiota showed progressive changes including selective enrichment of specific communities and lowered community richness and diversity during prolonged oral treatment with PM. Compared to control mice, the gut microbiota of PM-treated mice had significantly higher abundance of Verrucomicrobia and lower abundance of Firmicutes. Specific taxa that were significantly more abundant in PM-treated mice include Akkermansia muciniphila and several Bacteroides members. Predictive functional analysis revealed overrepresentation of carbohydrate metabolism function in the fecal microbiota of PM recipients compared to controls, and this function was linked to Bacteroides spp. Prolonged pretreatment with PM not only diminished susceptibility to dextran sulfate sodium induced colitis severity, but also caused enhanced immune regulation. Overall, this study demonstrates the prebiotic properties of PM and the potential benefits of its prolonged oral consumption to gut health. MDPI 2020-07-23 /pmc/articles/PMC7468787/ /pubmed/32717991 http://dx.doi.org/10.3390/nu12082193 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Taylor, Harrison B.
Gudi, Radhika
Brown, Robert
Vasu, Chenthamarakshan
Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title_full Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title_fullStr Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title_full_unstemmed Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title_short Dynamics of Structural and Functional Changes in Gut Microbiota during Treatment with a Microalgal β-Glucan, Paramylon and the Impact on Gut Inflammation
title_sort dynamics of structural and functional changes in gut microbiota during treatment with a microalgal β-glucan, paramylon and the impact on gut inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468787/
https://www.ncbi.nlm.nih.gov/pubmed/32717991
http://dx.doi.org/10.3390/nu12082193
work_keys_str_mv AT taylorharrisonb dynamicsofstructuralandfunctionalchangesingutmicrobiotaduringtreatmentwithamicroalgalbglucanparamylonandtheimpactongutinflammation
AT gudiradhika dynamicsofstructuralandfunctionalchangesingutmicrobiotaduringtreatmentwithamicroalgalbglucanparamylonandtheimpactongutinflammation
AT brownrobert dynamicsofstructuralandfunctionalchangesingutmicrobiotaduringtreatmentwithamicroalgalbglucanparamylonandtheimpactongutinflammation
AT vasuchenthamarakshan dynamicsofstructuralandfunctionalchangesingutmicrobiotaduringtreatmentwithamicroalgalbglucanparamylonandtheimpactongutinflammation