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Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes

Doxorubicin (doxo) is an effective anticancer compound in several tumor types. However, as a consequence of oxidative stress induction and ROS overproduction, its high cardiotoxicity demands urgent attention. Vanillin possesses antioxidant, antiproliferative, antidepressant and anti-glycating proper...

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Autores principales: Sirangelo, Ivana, Sapio, Luigi, Ragone, Angela, Naviglio, Silvio, Iannuzzi, Clara, Barone, Daniela, Giordano, Antonio, Borriello, Margherita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468857/
https://www.ncbi.nlm.nih.gov/pubmed/32752227
http://dx.doi.org/10.3390/nu12082317
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author Sirangelo, Ivana
Sapio, Luigi
Ragone, Angela
Naviglio, Silvio
Iannuzzi, Clara
Barone, Daniela
Giordano, Antonio
Borriello, Margherita
author_facet Sirangelo, Ivana
Sapio, Luigi
Ragone, Angela
Naviglio, Silvio
Iannuzzi, Clara
Barone, Daniela
Giordano, Antonio
Borriello, Margherita
author_sort Sirangelo, Ivana
collection PubMed
description Doxorubicin (doxo) is an effective anticancer compound in several tumor types. However, as a consequence of oxidative stress induction and ROS overproduction, its high cardiotoxicity demands urgent attention. Vanillin possesses antioxidant, antiproliferative, antidepressant and anti-glycating properties. Therefore, we investigated the potential vanillin protective effects against doxo-induced cardiotoxicity in H9c2 cells. Using multiparametric approach, we demonstrated that vanillin restored both cell viability and damage in response to doxo exposure. Contextually, vanillin decreased sub-G1 appearance and caspase-3 and PARP1 activation, reducing the doxo-related apoptosis induction. From a mechanistic point of view, vanillin hindered doxo-induced ROS accumulation and impaired the ERK phosphorylation. Notably, besides the cardioprotective effects, vanillin did not counteract the doxo effectiveness in osteosarcoma cells. Taken together, our results suggest that vanillin ameliorates doxo-induced toxicity in H9c2 cells, opening new avenues for developing alternative therapeutic approaches to prevent the anthracycline-related cardiotoxicity and to improve the long-term outcome of antineoplastic treatment.
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spelling pubmed-74688572020-09-04 Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes Sirangelo, Ivana Sapio, Luigi Ragone, Angela Naviglio, Silvio Iannuzzi, Clara Barone, Daniela Giordano, Antonio Borriello, Margherita Nutrients Article Doxorubicin (doxo) is an effective anticancer compound in several tumor types. However, as a consequence of oxidative stress induction and ROS overproduction, its high cardiotoxicity demands urgent attention. Vanillin possesses antioxidant, antiproliferative, antidepressant and anti-glycating properties. Therefore, we investigated the potential vanillin protective effects against doxo-induced cardiotoxicity in H9c2 cells. Using multiparametric approach, we demonstrated that vanillin restored both cell viability and damage in response to doxo exposure. Contextually, vanillin decreased sub-G1 appearance and caspase-3 and PARP1 activation, reducing the doxo-related apoptosis induction. From a mechanistic point of view, vanillin hindered doxo-induced ROS accumulation and impaired the ERK phosphorylation. Notably, besides the cardioprotective effects, vanillin did not counteract the doxo effectiveness in osteosarcoma cells. Taken together, our results suggest that vanillin ameliorates doxo-induced toxicity in H9c2 cells, opening new avenues for developing alternative therapeutic approaches to prevent the anthracycline-related cardiotoxicity and to improve the long-term outcome of antineoplastic treatment. MDPI 2020-08-01 /pmc/articles/PMC7468857/ /pubmed/32752227 http://dx.doi.org/10.3390/nu12082317 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sirangelo, Ivana
Sapio, Luigi
Ragone, Angela
Naviglio, Silvio
Iannuzzi, Clara
Barone, Daniela
Giordano, Antonio
Borriello, Margherita
Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title_full Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title_fullStr Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title_full_unstemmed Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title_short Vanillin Prevents Doxorubicin-Induced Apoptosis and Oxidative Stress in Rat H9c2 Cardiomyocytes
title_sort vanillin prevents doxorubicin-induced apoptosis and oxidative stress in rat h9c2 cardiomyocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468857/
https://www.ncbi.nlm.nih.gov/pubmed/32752227
http://dx.doi.org/10.3390/nu12082317
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