Cargando…

Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids

Axillary osmidrosis (AO) is a common chronic skin condition characterized by unpleasant body odors emanating from the armpits, and its aetiology is not fully understood. AO can seriously impair the psychosocial well-being of the affected individuals; however, no causal therapy has been established f...

Descripción completa

Detalles Bibliográficos
Autores principales: Saito, Hiroki, Toyoda, Yu, Hirata, Hiroshi, Ota-Kontani, Ami, Tsuchiya, Youichi, Takada, Tappei, Suzuki, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468911/
https://www.ncbi.nlm.nih.gov/pubmed/32824087
http://dx.doi.org/10.3390/nu12082452
_version_ 1783578313352019968
author Saito, Hiroki
Toyoda, Yu
Hirata, Hiroshi
Ota-Kontani, Ami
Tsuchiya, Youichi
Takada, Tappei
Suzuki, Hiroshi
author_facet Saito, Hiroki
Toyoda, Yu
Hirata, Hiroshi
Ota-Kontani, Ami
Tsuchiya, Youichi
Takada, Tappei
Suzuki, Hiroshi
author_sort Saito, Hiroki
collection PubMed
description Axillary osmidrosis (AO) is a common chronic skin condition characterized by unpleasant body odors emanating from the armpits, and its aetiology is not fully understood. AO can seriously impair the psychosocial well-being of the affected individuals; however, no causal therapy has been established for it other than surgical treatment. Recent studies have revealed that human ATP-binding cassette transporter C11 (ABCC11) is an AO risk factor when it is expressed in the axillary apocrine glands—the sources of the offensive odors. Hence, identifying safe ways to inhibit ABCC11 may offer a breakthrough in treating AO. We herein screened for ABCC11-inhibitory activities in 34 natural products derived from plants cultivated for human consumption using an in vitro assay system to measure the ABCC11-mediated transport of radiolabeled dehydroepiandrosterone sulfate (DHEA-S—an ABCC11 substrate). The water extract of soybean (Glycine max) was found to exhibit the strongest transport inhibition. From this extract, via a fractionation approach, we successfully isolated and identified genistein, a soy isoflavone, as a novel ABCC11 inhibitor with a half-maximal inhibitory concentration value of 61.5 μM. Furthermore, we examined the effects of other dietary flavonoids on the ABCC11-mediated DHEA-S transport to uncover the effects of these phytochemicals on ABCC11 function. While further human studies are needed, our findings here about the natural compounds will help develop a non-surgical therapy for AO.
format Online
Article
Text
id pubmed-7468911
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74689112020-09-04 Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids Saito, Hiroki Toyoda, Yu Hirata, Hiroshi Ota-Kontani, Ami Tsuchiya, Youichi Takada, Tappei Suzuki, Hiroshi Nutrients Article Axillary osmidrosis (AO) is a common chronic skin condition characterized by unpleasant body odors emanating from the armpits, and its aetiology is not fully understood. AO can seriously impair the psychosocial well-being of the affected individuals; however, no causal therapy has been established for it other than surgical treatment. Recent studies have revealed that human ATP-binding cassette transporter C11 (ABCC11) is an AO risk factor when it is expressed in the axillary apocrine glands—the sources of the offensive odors. Hence, identifying safe ways to inhibit ABCC11 may offer a breakthrough in treating AO. We herein screened for ABCC11-inhibitory activities in 34 natural products derived from plants cultivated for human consumption using an in vitro assay system to measure the ABCC11-mediated transport of radiolabeled dehydroepiandrosterone sulfate (DHEA-S—an ABCC11 substrate). The water extract of soybean (Glycine max) was found to exhibit the strongest transport inhibition. From this extract, via a fractionation approach, we successfully isolated and identified genistein, a soy isoflavone, as a novel ABCC11 inhibitor with a half-maximal inhibitory concentration value of 61.5 μM. Furthermore, we examined the effects of other dietary flavonoids on the ABCC11-mediated DHEA-S transport to uncover the effects of these phytochemicals on ABCC11 function. While further human studies are needed, our findings here about the natural compounds will help develop a non-surgical therapy for AO. MDPI 2020-08-14 /pmc/articles/PMC7468911/ /pubmed/32824087 http://dx.doi.org/10.3390/nu12082452 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saito, Hiroki
Toyoda, Yu
Hirata, Hiroshi
Ota-Kontani, Ami
Tsuchiya, Youichi
Takada, Tappei
Suzuki, Hiroshi
Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title_full Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title_fullStr Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title_full_unstemmed Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title_short Soy Isoflavone Genistein Inhibits an Axillary Osmidrosis Risk Factor ABCC11: In Vitro Screening and Fractional Approach for ABCC11-Inhibitory Activities in Plant Extracts and Dietary Flavonoids
title_sort soy isoflavone genistein inhibits an axillary osmidrosis risk factor abcc11: in vitro screening and fractional approach for abcc11-inhibitory activities in plant extracts and dietary flavonoids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468911/
https://www.ncbi.nlm.nih.gov/pubmed/32824087
http://dx.doi.org/10.3390/nu12082452
work_keys_str_mv AT saitohiroki soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT toyodayu soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT hiratahiroshi soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT otakontaniami soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT tsuchiyayouichi soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT takadatappei soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids
AT suzukihiroshi soyisoflavonegenisteininhibitsanaxillaryosmidrosisriskfactorabcc11invitroscreeningandfractionalapproachforabcc11inhibitoryactivitiesinplantextractsanddietaryflavonoids