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Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise

Background: Branched-chain amino acids (BCAAs: leucine, isoleucine, valine) account for 35% of skeletal muscle essential amino acids (AAs). As such, they must be provided in the diet to support peptide synthesis and inhibit protein breakdown. Although substantial evidence has been collected about th...

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Autores principales: Mantuano, Paola, Bianchini, Gianluca, Cappellari, Ornella, Boccanegra, Brigida, Conte, Elena, Sanarica, Francesca, Mele, Antonietta, Camerino, Giulia M., Brandolini, Laura, Allegretti, Marcello, De Bellis, Michela, Aramini, Andrea, De Luca, Annamaria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468919/
https://www.ncbi.nlm.nih.gov/pubmed/32751732
http://dx.doi.org/10.3390/nu12082295
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author Mantuano, Paola
Bianchini, Gianluca
Cappellari, Ornella
Boccanegra, Brigida
Conte, Elena
Sanarica, Francesca
Mele, Antonietta
Camerino, Giulia M.
Brandolini, Laura
Allegretti, Marcello
De Bellis, Michela
Aramini, Andrea
De Luca, Annamaria
author_facet Mantuano, Paola
Bianchini, Gianluca
Cappellari, Ornella
Boccanegra, Brigida
Conte, Elena
Sanarica, Francesca
Mele, Antonietta
Camerino, Giulia M.
Brandolini, Laura
Allegretti, Marcello
De Bellis, Michela
Aramini, Andrea
De Luca, Annamaria
author_sort Mantuano, Paola
collection PubMed
description Background: Branched-chain amino acids (BCAAs: leucine, isoleucine, valine) account for 35% of skeletal muscle essential amino acids (AAs). As such, they must be provided in the diet to support peptide synthesis and inhibit protein breakdown. Although substantial evidence has been collected about the potential usefulness of BCAAs in supporting muscle function and structure, dietary supplements containing BCAAs alone may not be effective in controlling muscle protein turnover, due to the rate-limiting bioavailability of other AAs involved in BCAAs metabolism. Methods: We aimed to evaluate the in vivo/ex vivo effects of a 4-week treatment with an oral formulation containing BCAAs alone (2:1:1) on muscle function, structure, and metabolism in a murine model of physiological exercise, which was compared to three modified formulations combining BCAAs with increasing concentrations of L-Alanine (ALA), an AA controlling BCAAs catabolism. Results: A preliminary pharmacokinetic study confirmed the ability of ALA to boost up BCAAs bioavailability. After 4 weeks, mix 2 (BCAAs + 2ALA) had the best protective effect on mice force and fatigability, as well as on muscle morphology and metabolic indices. Conclusion: Our study corroborates the use of BCAAs + ALA to support muscle health during physiological exercise, underlining how the relative BCAAs/ALA ratio is important to control BCAAs distribution.
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spelling pubmed-74689192020-09-04 Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise Mantuano, Paola Bianchini, Gianluca Cappellari, Ornella Boccanegra, Brigida Conte, Elena Sanarica, Francesca Mele, Antonietta Camerino, Giulia M. Brandolini, Laura Allegretti, Marcello De Bellis, Michela Aramini, Andrea De Luca, Annamaria Nutrients Article Background: Branched-chain amino acids (BCAAs: leucine, isoleucine, valine) account for 35% of skeletal muscle essential amino acids (AAs). As such, they must be provided in the diet to support peptide synthesis and inhibit protein breakdown. Although substantial evidence has been collected about the potential usefulness of BCAAs in supporting muscle function and structure, dietary supplements containing BCAAs alone may not be effective in controlling muscle protein turnover, due to the rate-limiting bioavailability of other AAs involved in BCAAs metabolism. Methods: We aimed to evaluate the in vivo/ex vivo effects of a 4-week treatment with an oral formulation containing BCAAs alone (2:1:1) on muscle function, structure, and metabolism in a murine model of physiological exercise, which was compared to three modified formulations combining BCAAs with increasing concentrations of L-Alanine (ALA), an AA controlling BCAAs catabolism. Results: A preliminary pharmacokinetic study confirmed the ability of ALA to boost up BCAAs bioavailability. After 4 weeks, mix 2 (BCAAs + 2ALA) had the best protective effect on mice force and fatigability, as well as on muscle morphology and metabolic indices. Conclusion: Our study corroborates the use of BCAAs + ALA to support muscle health during physiological exercise, underlining how the relative BCAAs/ALA ratio is important to control BCAAs distribution. MDPI 2020-07-30 /pmc/articles/PMC7468919/ /pubmed/32751732 http://dx.doi.org/10.3390/nu12082295 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mantuano, Paola
Bianchini, Gianluca
Cappellari, Ornella
Boccanegra, Brigida
Conte, Elena
Sanarica, Francesca
Mele, Antonietta
Camerino, Giulia M.
Brandolini, Laura
Allegretti, Marcello
De Bellis, Michela
Aramini, Andrea
De Luca, Annamaria
Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title_full Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title_fullStr Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title_full_unstemmed Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title_short Ergogenic Effect of BCAAs and L-Alanine Supplementation: Proof-of-Concept Study in a Murine Model of Physiological Exercise
title_sort ergogenic effect of bcaas and l-alanine supplementation: proof-of-concept study in a murine model of physiological exercise
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7468919/
https://www.ncbi.nlm.nih.gov/pubmed/32751732
http://dx.doi.org/10.3390/nu12082295
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