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Simple Colorimetric Assay for Vibrio parahaemolyticus Detection Using Aptamer-Functionalized Nanoparticles
[Image: see text] Simple, rapid, and sensitive screening methods are the key to prevent and control the spread of foodborne diseases. In this study, a simple visual colorimetric assay using magnetic nanoparticles (MNPs) and gold nanoparticles (AuNPs) was developed for the detection of Vibrio parahae...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469129/ https://www.ncbi.nlm.nih.gov/pubmed/32905329 http://dx.doi.org/10.1021/acsomega.0c01795 |
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author | Sadsri, Varunee Trakulsujaritchok, Thanida Tangwattanachuleeporn, Marut Hoven, Voravee P. Na Nongkhai, Piyaporn |
author_facet | Sadsri, Varunee Trakulsujaritchok, Thanida Tangwattanachuleeporn, Marut Hoven, Voravee P. Na Nongkhai, Piyaporn |
author_sort | Sadsri, Varunee |
collection | PubMed |
description | [Image: see text] Simple, rapid, and sensitive screening methods are the key to prevent and control the spread of foodborne diseases. In this study, a simple visual colorimetric assay using magnetic nanoparticles (MNPs) and gold nanoparticles (AuNPs) was developed for the detection of Vibrio parahaemolyticus. First, the aptamer responding to V. parahaemolyticus was conjugated onto the surface of MNPs and used as a specific magnetic separator. In addition, the aptamer was also immobilized on the surface of AuNPs and used as a colorimetric detector. In the presence of V. parahaemolyticus, a sandwich structure of MNP–aptamer–bacteria–aptamer–AuNPs is formed through specific recognition of the aptamer and V. parahaemolyticus. The magnetic separation technique was then applied to generate a detection signal. Owing to the optical properties of AuNPs, a visual signal could be observed, resulting in an instrument-free colorimetric detection. Under optimal conditions, this assay shows a linear response toward V. parahaemolyticus concentration through the range of 10–10(6) cfu/mL, with a limit of detection of 2.4 cfu/mL. This method was also successfully applied for V. parahaemolyticus detection in spiked raw shrimp samples. |
format | Online Article Text |
id | pubmed-7469129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74691292020-09-04 Simple Colorimetric Assay for Vibrio parahaemolyticus Detection Using Aptamer-Functionalized Nanoparticles Sadsri, Varunee Trakulsujaritchok, Thanida Tangwattanachuleeporn, Marut Hoven, Voravee P. Na Nongkhai, Piyaporn ACS Omega [Image: see text] Simple, rapid, and sensitive screening methods are the key to prevent and control the spread of foodborne diseases. In this study, a simple visual colorimetric assay using magnetic nanoparticles (MNPs) and gold nanoparticles (AuNPs) was developed for the detection of Vibrio parahaemolyticus. First, the aptamer responding to V. parahaemolyticus was conjugated onto the surface of MNPs and used as a specific magnetic separator. In addition, the aptamer was also immobilized on the surface of AuNPs and used as a colorimetric detector. In the presence of V. parahaemolyticus, a sandwich structure of MNP–aptamer–bacteria–aptamer–AuNPs is formed through specific recognition of the aptamer and V. parahaemolyticus. The magnetic separation technique was then applied to generate a detection signal. Owing to the optical properties of AuNPs, a visual signal could be observed, resulting in an instrument-free colorimetric detection. Under optimal conditions, this assay shows a linear response toward V. parahaemolyticus concentration through the range of 10–10(6) cfu/mL, with a limit of detection of 2.4 cfu/mL. This method was also successfully applied for V. parahaemolyticus detection in spiked raw shrimp samples. American Chemical Society 2020-08-18 /pmc/articles/PMC7469129/ /pubmed/32905329 http://dx.doi.org/10.1021/acsomega.0c01795 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Sadsri, Varunee Trakulsujaritchok, Thanida Tangwattanachuleeporn, Marut Hoven, Voravee P. Na Nongkhai, Piyaporn Simple Colorimetric Assay for Vibrio parahaemolyticus Detection Using Aptamer-Functionalized Nanoparticles |
title | Simple Colorimetric Assay for Vibrio
parahaemolyticus Detection Using Aptamer-Functionalized
Nanoparticles |
title_full | Simple Colorimetric Assay for Vibrio
parahaemolyticus Detection Using Aptamer-Functionalized
Nanoparticles |
title_fullStr | Simple Colorimetric Assay for Vibrio
parahaemolyticus Detection Using Aptamer-Functionalized
Nanoparticles |
title_full_unstemmed | Simple Colorimetric Assay for Vibrio
parahaemolyticus Detection Using Aptamer-Functionalized
Nanoparticles |
title_short | Simple Colorimetric Assay for Vibrio
parahaemolyticus Detection Using Aptamer-Functionalized
Nanoparticles |
title_sort | simple colorimetric assay for vibrio
parahaemolyticus detection using aptamer-functionalized
nanoparticles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469129/ https://www.ncbi.nlm.nih.gov/pubmed/32905329 http://dx.doi.org/10.1021/acsomega.0c01795 |
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