Functional Variation in Dipteran Gut Bacterial Communities in Relation to Their Diet, Life Cycle Stage and Habitat

SIMPLE SUMMARY: Like in many other organisms, the guts of insects are full with many different bacteria. These bacteria can help their hosts to overcome toxic diets or can boost their resistance to pathogens. We were curious to learn which factors determine the composition of gut bacterial communities (GBCs) in true flies and mosquitoes, which belong to the order Diptera. We searched for research papers reporting on GBCs in these insects. Using these published data, we investigated whether the GBCs are species-specific, or whether they are determined by the diet, life stage or environment of the host insect. We found that the GBCs in larvae and adults of the same insect species can be very different. Insects on similar diets did not necessarily show similar GBCs. This made us conclude that GBCs are mostly life stage-specific. However, we found that the number of data papers we could use is limited; more data are needed to strengthen our conclusion. Lastly, novel DNA technologies can show ‘who is there’ in GBCs. At the same time, we lack knowledge on the exact function of gut bacteria. Obtaining more knowledge on the function of GBCs may help to design sustainable pest control measures. ABSTRACT: True flies and mosquitos (Diptera) live in habitats and consume diets that pose specific demands on their gut bacterial communities (GBCs). Due to diet specializations, dipterans may have highly diverse and species-specific GBCs. Dipterans are also confronted with changes in habitat and food sources over their lifetime, especially during life history processes (molting, metamorphosis). This may prevent the development of a constant species- or diet-specific GBC. Some dipterans are vectors of several human pathogens (e.g., malaria), which interact with GBCs. In this review, we explore the dynamics that shape GBC composition in some Diptera species on the basis of published datasets of GBCs. We thereby focus on the effects of diet, habitats, and life cycle stages as sources of variation in GBC composition. The GBCs reported were more stage-specific than species- or diet-specific. Even though the presence of GBCs has a large impact on the performance of their hosts, the exact functions of GBCs and their interactions with other organisms are still largely unknown, mainly due to the low number of studies to date. Increasing our knowledge on dipteran GBCs will help to design pest management strategies for the reduction of insecticide resistance, as well as for human pathogen control.