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The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests

Insect pests reduce global crop yields by up to 20%, but the most effective control measures are currently based on environmentally hazardous chemical pesticides. An alternative, ecologically beneficial pest-management strategy involves the use of microbial pathogens (or active compounds and extract...

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Autores principales: Hiebert, Nils, Kessel, Tobias, Skaljac, Marisa, Spohn, Marius, Vilcinskas, Andreas, Lee, Kwang-Zin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469177/
https://www.ncbi.nlm.nih.gov/pubmed/32722463
http://dx.doi.org/10.3390/insects11080471
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author Hiebert, Nils
Kessel, Tobias
Skaljac, Marisa
Spohn, Marius
Vilcinskas, Andreas
Lee, Kwang-Zin
author_facet Hiebert, Nils
Kessel, Tobias
Skaljac, Marisa
Spohn, Marius
Vilcinskas, Andreas
Lee, Kwang-Zin
author_sort Hiebert, Nils
collection PubMed
description Insect pests reduce global crop yields by up to 20%, but the most effective control measures are currently based on environmentally hazardous chemical pesticides. An alternative, ecologically beneficial pest-management strategy involves the use of microbial pathogens (or active compounds and extracts derived from them) that naturally target selected insect pests. A novel strain of the bacterium Leuconostoc pseudomesenteroides showed promising activity in our preliminary tests. Here, we investigated its effects in more detail, focusing on drosophilid and aphid pests by testing the survival of two species representing the family Drosophilidae (Drosophila suzukii and D. melanogaster) and one representing the family Aphididae (Acyrthosiphon pisum). We used oral and septic infection models to administer living bacteria or cell-free extracts to adult flies and aphid nymphs. We found that infection with living bacteria significantly reduced the survival of our insect models, whereas the administration of cell-free extracts had a significant effect only in aphids. These results confirm that L. pseudomesenteroides has potential as a new biocontrol agent for sustainable pest management.
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spelling pubmed-74691772020-09-17 The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests Hiebert, Nils Kessel, Tobias Skaljac, Marisa Spohn, Marius Vilcinskas, Andreas Lee, Kwang-Zin Insects Article Insect pests reduce global crop yields by up to 20%, but the most effective control measures are currently based on environmentally hazardous chemical pesticides. An alternative, ecologically beneficial pest-management strategy involves the use of microbial pathogens (or active compounds and extracts derived from them) that naturally target selected insect pests. A novel strain of the bacterium Leuconostoc pseudomesenteroides showed promising activity in our preliminary tests. Here, we investigated its effects in more detail, focusing on drosophilid and aphid pests by testing the survival of two species representing the family Drosophilidae (Drosophila suzukii and D. melanogaster) and one representing the family Aphididae (Acyrthosiphon pisum). We used oral and septic infection models to administer living bacteria or cell-free extracts to adult flies and aphid nymphs. We found that infection with living bacteria significantly reduced the survival of our insect models, whereas the administration of cell-free extracts had a significant effect only in aphids. These results confirm that L. pseudomesenteroides has potential as a new biocontrol agent for sustainable pest management. MDPI 2020-07-25 /pmc/articles/PMC7469177/ /pubmed/32722463 http://dx.doi.org/10.3390/insects11080471 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hiebert, Nils
Kessel, Tobias
Skaljac, Marisa
Spohn, Marius
Vilcinskas, Andreas
Lee, Kwang-Zin
The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title_full The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title_fullStr The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title_full_unstemmed The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title_short The Gram-Positive Bacterium Leuconostoc pseudomesenteroides Shows Insecticidal Activity against Drosophilid and Aphid Pests
title_sort gram-positive bacterium leuconostoc pseudomesenteroides shows insecticidal activity against drosophilid and aphid pests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469177/
https://www.ncbi.nlm.nih.gov/pubmed/32722463
http://dx.doi.org/10.3390/insects11080471
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