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Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions

Bees collect pollen from flowers for their offspring, and by doing so contribute critical pollination services for our crops and ecosystems. Unlike many managed bee species, wild bees are thought to obtain much of their microbiome from the environment. However, we know surprisingly little about what...

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Autores principales: Dew, Rebecca M., McFrederick, Quinn S., Rehan, Sandra M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469187/
https://www.ncbi.nlm.nih.gov/pubmed/32759653
http://dx.doi.org/10.3390/insects11080499
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author Dew, Rebecca M.
McFrederick, Quinn S.
Rehan, Sandra M.
author_facet Dew, Rebecca M.
McFrederick, Quinn S.
Rehan, Sandra M.
author_sort Dew, Rebecca M.
collection PubMed
description Bees collect pollen from flowers for their offspring, and by doing so contribute critical pollination services for our crops and ecosystems. Unlike many managed bee species, wild bees are thought to obtain much of their microbiome from the environment. However, we know surprisingly little about what plant species bees visit and the microbes associated with the collected pollen. Here, we addressed the hypothesis that the pollen and microbial components of bee diets would change across the range of the bee, by amplicon sequencing pollen provisions of a widespread small carpenter bee, Ceratina calcarata, across three populations. Ceratina calcarata was found to use a diversity of floral resources across its range, but the bacterial genera associated with pollen provisions were very consistent. Acinetobacter, Erwinia, Lactobacillus, Sodalis, Sphingomonas and Wolbachia were among the top ten bacterial genera across all sites. Ceratina calcarata uses both raspberry (Rubus) and sumac (Rhus) stems as nesting substrates, however nests within these plants showed no preference for host plant pollen. Significant correlations in plant and bacterial co-occurrence differed between sites, indicating that many of the most common bacterial genera have either regional or transitory floral associations. This range-wide study suggests microbes present in brood provisions are conserved within a bee species, rather than mediated by climate or pollen composition. Moving forward, this has important implications for how these core bacteria affect larval health and whether these functions vary across space and diet. These data increase our understanding of how pollinators interact with and adjust to their changing environment.
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spelling pubmed-74691872020-09-17 Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions Dew, Rebecca M. McFrederick, Quinn S. Rehan, Sandra M. Insects Article Bees collect pollen from flowers for their offspring, and by doing so contribute critical pollination services for our crops and ecosystems. Unlike many managed bee species, wild bees are thought to obtain much of their microbiome from the environment. However, we know surprisingly little about what plant species bees visit and the microbes associated with the collected pollen. Here, we addressed the hypothesis that the pollen and microbial components of bee diets would change across the range of the bee, by amplicon sequencing pollen provisions of a widespread small carpenter bee, Ceratina calcarata, across three populations. Ceratina calcarata was found to use a diversity of floral resources across its range, but the bacterial genera associated with pollen provisions were very consistent. Acinetobacter, Erwinia, Lactobacillus, Sodalis, Sphingomonas and Wolbachia were among the top ten bacterial genera across all sites. Ceratina calcarata uses both raspberry (Rubus) and sumac (Rhus) stems as nesting substrates, however nests within these plants showed no preference for host plant pollen. Significant correlations in plant and bacterial co-occurrence differed between sites, indicating that many of the most common bacterial genera have either regional or transitory floral associations. This range-wide study suggests microbes present in brood provisions are conserved within a bee species, rather than mediated by climate or pollen composition. Moving forward, this has important implications for how these core bacteria affect larval health and whether these functions vary across space and diet. These data increase our understanding of how pollinators interact with and adjust to their changing environment. MDPI 2020-08-04 /pmc/articles/PMC7469187/ /pubmed/32759653 http://dx.doi.org/10.3390/insects11080499 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dew, Rebecca M.
McFrederick, Quinn S.
Rehan, Sandra M.
Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title_full Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title_fullStr Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title_full_unstemmed Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title_short Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions
title_sort diverse diets with consistent core microbiome in wild bee pollen provisions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469187/
https://www.ncbi.nlm.nih.gov/pubmed/32759653
http://dx.doi.org/10.3390/insects11080499
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