Cargando…
Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study
[Image: see text] Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated the performance of vascular patches electrospun using a blend of polycaprolactone (PCL) and polyhydroxyb...
Autores principales: | , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469394/ https://www.ncbi.nlm.nih.gov/pubmed/32905385 http://dx.doi.org/10.1021/acsomega.0c02593 |
_version_ | 1783578418048139264 |
---|---|
author | Sevostianova, Viktoriia V. Antonova, Larisa V. Mironov, Andrey V. Yuzhalin, Arseniy E. Silnikov, Vladimir N. Glushkova, Tatiana V. Godovikova, Tatyana S. Krivkina, Evgeniya O. Bolbasov, Evgeniy Akentyeva, Tatiana N. Khanova, Mariam Yu. Matveeva, Vera G. Velikanova, Elena A. Tarasov, Roman S. Barbarash, Leonid S. |
author_facet | Sevostianova, Viktoriia V. Antonova, Larisa V. Mironov, Andrey V. Yuzhalin, Arseniy E. Silnikov, Vladimir N. Glushkova, Tatiana V. Godovikova, Tatyana S. Krivkina, Evgeniya O. Bolbasov, Evgeniy Akentyeva, Tatiana N. Khanova, Mariam Yu. Matveeva, Vera G. Velikanova, Elena A. Tarasov, Roman S. Barbarash, Leonid S. |
author_sort | Sevostianova, Viktoriia V. |
collection | PubMed |
description | [Image: see text] Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated the performance of vascular patches electrospun using a blend of polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV) and additionally modified with RGDK, AhRGD, and c[RGDFK] peptides using 1,6-hexamethylenediamine or 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) linkers. We examined mechanical properties and hemocompatibility of resulting patches before implanting them in rat abdominal aortas to assess their performance in vivo. Patches were explanted 1, 3, 6, and 12 months postoperation followed by histological and immunofluorescence analyses. Patches manufactured from the human internal mammary artery or commercially available KemPeriplas-Neo xenopericardial patches were used as a control. The tensile strength and F(max) of KemPeriplas-Neo patches were 4- and 16.7-times higher than those made of human internal mammary artery, respectively. Both RGD-modified and unmodified PHBV/PCL patches demonstrated properties similar to a human internal mammary artery patch. Regardless of RGD modification, experimental PHBV/PCL patches displayed fewer lysed red blood cells and resulted in milder platelet aggregation than KemPeriplas-Neo patches. Xenopericardial patches failed to form an endothelial layer in vivo and were prone to calcification. By contrast, TTDDA/RGDK-modified biodegradable patches demonstrated a resistance to calcification. Modification by TTDDA/RGDK and TTDDA/c[RGDFK] facilitated the formation of neovasculature upon the implantation in vivo. |
format | Online Article Text |
id | pubmed-7469394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74693942020-09-04 Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study Sevostianova, Viktoriia V. Antonova, Larisa V. Mironov, Andrey V. Yuzhalin, Arseniy E. Silnikov, Vladimir N. Glushkova, Tatiana V. Godovikova, Tatyana S. Krivkina, Evgeniya O. Bolbasov, Evgeniy Akentyeva, Tatiana N. Khanova, Mariam Yu. Matveeva, Vera G. Velikanova, Elena A. Tarasov, Roman S. Barbarash, Leonid S. ACS Omega [Image: see text] Modification by Arg-Gly-Asp (RGD) peptides is a promising approach to improve the biocompatibility of biodegradable vascular patches for arteriotomy. In this study, we evaluated the performance of vascular patches electrospun using a blend of polycaprolactone (PCL) and polyhydroxybutyrate/valerate (PHBV) and additionally modified with RGDK, AhRGD, and c[RGDFK] peptides using 1,6-hexamethylenediamine or 4,7,10-trioxa-1,13-tridecanediamine (TTDDA) linkers. We examined mechanical properties and hemocompatibility of resulting patches before implanting them in rat abdominal aortas to assess their performance in vivo. Patches were explanted 1, 3, 6, and 12 months postoperation followed by histological and immunofluorescence analyses. Patches manufactured from the human internal mammary artery or commercially available KemPeriplas-Neo xenopericardial patches were used as a control. The tensile strength and F(max) of KemPeriplas-Neo patches were 4- and 16.7-times higher than those made of human internal mammary artery, respectively. Both RGD-modified and unmodified PHBV/PCL patches demonstrated properties similar to a human internal mammary artery patch. Regardless of RGD modification, experimental PHBV/PCL patches displayed fewer lysed red blood cells and resulted in milder platelet aggregation than KemPeriplas-Neo patches. Xenopericardial patches failed to form an endothelial layer in vivo and were prone to calcification. By contrast, TTDDA/RGDK-modified biodegradable patches demonstrated a resistance to calcification. Modification by TTDDA/RGDK and TTDDA/c[RGDFK] facilitated the formation of neovasculature upon the implantation in vivo. American Chemical Society 2020-08-19 /pmc/articles/PMC7469394/ /pubmed/32905385 http://dx.doi.org/10.1021/acsomega.0c02593 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Sevostianova, Viktoriia V. Antonova, Larisa V. Mironov, Andrey V. Yuzhalin, Arseniy E. Silnikov, Vladimir N. Glushkova, Tatiana V. Godovikova, Tatyana S. Krivkina, Evgeniya O. Bolbasov, Evgeniy Akentyeva, Tatiana N. Khanova, Mariam Yu. Matveeva, Vera G. Velikanova, Elena A. Tarasov, Roman S. Barbarash, Leonid S. Biodegradable Patches for Arterial Reconstruction Modified with RGD Peptides: Results of an Experimental Study |
title | Biodegradable Patches for Arterial Reconstruction
Modified with RGD Peptides: Results of an Experimental Study |
title_full | Biodegradable Patches for Arterial Reconstruction
Modified with RGD Peptides: Results of an Experimental Study |
title_fullStr | Biodegradable Patches for Arterial Reconstruction
Modified with RGD Peptides: Results of an Experimental Study |
title_full_unstemmed | Biodegradable Patches for Arterial Reconstruction
Modified with RGD Peptides: Results of an Experimental Study |
title_short | Biodegradable Patches for Arterial Reconstruction
Modified with RGD Peptides: Results of an Experimental Study |
title_sort | biodegradable patches for arterial reconstruction
modified with rgd peptides: results of an experimental study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469394/ https://www.ncbi.nlm.nih.gov/pubmed/32905385 http://dx.doi.org/10.1021/acsomega.0c02593 |
work_keys_str_mv | AT sevostianovaviktoriiav biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT antonovalarisav biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT mironovandreyv biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT yuzhalinarseniye biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT silnikovvladimirn biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT glushkovatatianav biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT godovikovatatyanas biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT krivkinaevgeniyao biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT bolbasovevgeniy biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT akentyevatatianan biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT khanovamariamyu biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT matveevaverag biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT velikanovaelenaa biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT tarasovromans biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy AT barbarashleonids biodegradablepatchesforarterialreconstructionmodifiedwithrgdpeptidesresultsofanexperimentalstudy |