Cargando…
Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis)
The arms race between plants and viruses never ceases. Chinese cabbage, an important type of Brassica vegetable crop, is vulnerable to plant virus infection, especially to Turnip mosaic virus (TuMV). To better examine the molecular mechanisms behind the virus infection, we conducted the correlation...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469979/ https://www.ncbi.nlm.nih.gov/pubmed/32973883 http://dx.doi.org/10.3389/fgene.2020.00963 |
_version_ | 1783578497100283904 |
---|---|
author | Lyu, Shanwu Gao, Liwei Zhang, Rujia Zhang, Changwei Hou, Xilin |
author_facet | Lyu, Shanwu Gao, Liwei Zhang, Rujia Zhang, Changwei Hou, Xilin |
author_sort | Lyu, Shanwu |
collection | PubMed |
description | The arms race between plants and viruses never ceases. Chinese cabbage, an important type of Brassica vegetable crop, is vulnerable to plant virus infection, especially to Turnip mosaic virus (TuMV). To better examine the molecular mechanisms behind the virus infection, we conducted the correlation analysis of RNA-Seq and quantitative iTRAQ-LC-MS/MS in TuMV-infected and in healthy Chinese cabbage leaves. There were 757 differentially expressed genes and 75 differentially expressed proteins that were screened in Chinese cabbage plants infected with TuMV. These genes were enriched in many pathways, and among them, the plant hormone signal transduction, plant-pathogen interaction, and protein processing in the endoplasmic reticulum pathways were suggested to be closely related pathways. The correlation analysis between RNA-Seq and quantitative iTRAQ-LC-MS/MS was then further explored. Finally, we obtained a preliminary network of several candidate genes associated with TuMV infection, and we found that they mainly belonged to calcium signaling pathways, heat shock proteins, WRKY transcription factors, and non-specific lipid transfer proteins. These results may lead to a better understanding of antiviral mechanisms and of disease-resistant breeding. |
format | Online Article Text |
id | pubmed-7469979 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74699792020-09-23 Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) Lyu, Shanwu Gao, Liwei Zhang, Rujia Zhang, Changwei Hou, Xilin Front Genet Genetics The arms race between plants and viruses never ceases. Chinese cabbage, an important type of Brassica vegetable crop, is vulnerable to plant virus infection, especially to Turnip mosaic virus (TuMV). To better examine the molecular mechanisms behind the virus infection, we conducted the correlation analysis of RNA-Seq and quantitative iTRAQ-LC-MS/MS in TuMV-infected and in healthy Chinese cabbage leaves. There were 757 differentially expressed genes and 75 differentially expressed proteins that were screened in Chinese cabbage plants infected with TuMV. These genes were enriched in many pathways, and among them, the plant hormone signal transduction, plant-pathogen interaction, and protein processing in the endoplasmic reticulum pathways were suggested to be closely related pathways. The correlation analysis between RNA-Seq and quantitative iTRAQ-LC-MS/MS was then further explored. Finally, we obtained a preliminary network of several candidate genes associated with TuMV infection, and we found that they mainly belonged to calcium signaling pathways, heat shock proteins, WRKY transcription factors, and non-specific lipid transfer proteins. These results may lead to a better understanding of antiviral mechanisms and of disease-resistant breeding. Frontiers Media S.A. 2020-08-20 /pmc/articles/PMC7469979/ /pubmed/32973883 http://dx.doi.org/10.3389/fgene.2020.00963 Text en Copyright © 2020 Lyu, Gao, Zhang, Zhang and Hou. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Lyu, Shanwu Gao, Liwei Zhang, Rujia Zhang, Changwei Hou, Xilin Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title | Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title_full | Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title_fullStr | Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title_full_unstemmed | Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title_short | Correlation Analysis of Expression Profile and Quantitative iTRAQ-LC-MS/MS Proteomics Reveals Resistance Mechanism Against TuMV in Chinese Cabbage (Brassica rapa ssp. pekinensis) |
title_sort | correlation analysis of expression profile and quantitative itraq-lc-ms/ms proteomics reveals resistance mechanism against tumv in chinese cabbage (brassica rapa ssp. pekinensis) |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7469979/ https://www.ncbi.nlm.nih.gov/pubmed/32973883 http://dx.doi.org/10.3389/fgene.2020.00963 |
work_keys_str_mv | AT lyushanwu correlationanalysisofexpressionprofileandquantitativeitraqlcmsmsproteomicsrevealsresistancemechanismagainsttumvinchinesecabbagebrassicarapassppekinensis AT gaoliwei correlationanalysisofexpressionprofileandquantitativeitraqlcmsmsproteomicsrevealsresistancemechanismagainsttumvinchinesecabbagebrassicarapassppekinensis AT zhangrujia correlationanalysisofexpressionprofileandquantitativeitraqlcmsmsproteomicsrevealsresistancemechanismagainsttumvinchinesecabbagebrassicarapassppekinensis AT zhangchangwei correlationanalysisofexpressionprofileandquantitativeitraqlcmsmsproteomicsrevealsresistancemechanismagainsttumvinchinesecabbagebrassicarapassppekinensis AT houxilin correlationanalysisofexpressionprofileandquantitativeitraqlcmsmsproteomicsrevealsresistancemechanismagainsttumvinchinesecabbagebrassicarapassppekinensis |