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The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus

AIM: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (S. aureus). Methodology: The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Labo...

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Autores principales: Parvekar, Prashik, Palaskar, Jayant, Metgud, Sandeep, Maria, Rahul, Dutta, Smita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470068/
https://www.ncbi.nlm.nih.gov/pubmed/32939454
http://dx.doi.org/10.1080/26415275.2020.1796674
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author Parvekar, Prashik
Palaskar, Jayant
Metgud, Sandeep
Maria, Rahul
Dutta, Smita
author_facet Parvekar, Prashik
Palaskar, Jayant
Metgud, Sandeep
Maria, Rahul
Dutta, Smita
author_sort Parvekar, Prashik
collection PubMed
description AIM: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (S. aureus). Methodology: The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Laboratory Standards Institute (CLSI). Different concentrations of silver nanoparticles were prepared, and MIC was calculated by tube macro-dilution method. The MBC was determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data were analyzed by ANOVA and Tukey’s post hoc test using SPSS software. RESULTS: The MIC and MBC of silver nanoparticles against S. aureus was found to be 0.625 mg/ml. CONCLUSION: The result obtained from this study shows that silver nanoparticles have potential bactericidal effects against S. aureus at a concentration of 0.625 mg/ml. Silver nanoparticles can be incorporated in the root canal medicaments, sealers and irrigants as it possess potent antimicrobial efficacy against S. aureus.
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spelling pubmed-74700682020-09-15 The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus Parvekar, Prashik Palaskar, Jayant Metgud, Sandeep Maria, Rahul Dutta, Smita Biomater Investig Dent Original Article AIM: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus (S. aureus). Methodology: The antimicrobial efficacy of the silver nanoparticles was determined by the standard methods of Clinical and Laboratory Standards Institute (CLSI). Different concentrations of silver nanoparticles were prepared, and MIC was calculated by tube macro-dilution method. The MBC was determined by the lowest concentration that kills 99.9% of the initial bacterial population. The data were analyzed by ANOVA and Tukey’s post hoc test using SPSS software. RESULTS: The MIC and MBC of silver nanoparticles against S. aureus was found to be 0.625 mg/ml. CONCLUSION: The result obtained from this study shows that silver nanoparticles have potential bactericidal effects against S. aureus at a concentration of 0.625 mg/ml. Silver nanoparticles can be incorporated in the root canal medicaments, sealers and irrigants as it possess potent antimicrobial efficacy against S. aureus. Taylor & Francis 2020-07-23 /pmc/articles/PMC7470068/ /pubmed/32939454 http://dx.doi.org/10.1080/26415275.2020.1796674 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Parvekar, Prashik
Palaskar, Jayant
Metgud, Sandeep
Maria, Rahul
Dutta, Smita
The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title_full The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title_fullStr The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title_full_unstemmed The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title_short The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus
title_sort minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of silver nanoparticles against staphylococcus aureus
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470068/
https://www.ncbi.nlm.nih.gov/pubmed/32939454
http://dx.doi.org/10.1080/26415275.2020.1796674
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