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Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro/in vivo study
This study aims to improve the anticancer activity of bovine lactoferrin through enhancing its stability by immobilization onto graphene oxide. Bovine lactoferrin was conjugated onto graphene oxide and the conjugation process was confirmed by FT-IR, SDS-PAGE, and UV spectrophotometry. Physical chara...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470100/ https://www.ncbi.nlm.nih.gov/pubmed/32812454 http://dx.doi.org/10.1080/10717544.2020.1809558 |
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author | Najmafshar, Azam Rostami, Mahboubeh Varshosaz, Jaleh Norouzian, Dariush Samsam Shariat, Seyed Ziyae Aldin |
author_facet | Najmafshar, Azam Rostami, Mahboubeh Varshosaz, Jaleh Norouzian, Dariush Samsam Shariat, Seyed Ziyae Aldin |
author_sort | Najmafshar, Azam |
collection | PubMed |
description | This study aims to improve the anticancer activity of bovine lactoferrin through enhancing its stability by immobilization onto graphene oxide. Bovine lactoferrin was conjugated onto graphene oxide and the conjugation process was confirmed by FT-IR, SDS-PAGE, and UV spectrophotometry. Physical characterization was performed by DLS analysis and atomic force microscopy. The cytotoxicity and cellular uptake of the final construct (CGO-PEG-bLF) was inspected on lung cancer TC-1 cells by MTT assay and flow cytometry/confocal microscopy. The anticancer mechanism of the CGO-PEG-bLF was studied by cell cycle analysis, apoptosis assay, and western blot technique. Finally, the anticancer activity of CGO-PEG-bLF was assessed in an animal model of lung cancer. Size and zeta potential of CGO-PEG-bLF was obtained in the optimum range. Compared with free bLF, more cytotoxic activity, cellular uptake and more survival time was obtained for CGO-PEG-bLF. CGO-PEG-bLF significantly inhibited tumor growth in the animal model. Cell cycle arrest and apoptosis were more induced by CGO-PEG-bLF. Moreover, exposure to CGO-PEG-bLF decreased the phospho-AKT and pro-Caspase 3 levels and increased the amount of cleaved caspase 3 in the treated cells. This study revealed the potential of CGO-PEG as a promising nanocarrier for enhancing the therapeutic efficacy of anticancer agents. |
format | Online Article Text |
id | pubmed-7470100 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-74701002020-09-15 Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro/in vivo study Najmafshar, Azam Rostami, Mahboubeh Varshosaz, Jaleh Norouzian, Dariush Samsam Shariat, Seyed Ziyae Aldin Drug Deliv Research Article This study aims to improve the anticancer activity of bovine lactoferrin through enhancing its stability by immobilization onto graphene oxide. Bovine lactoferrin was conjugated onto graphene oxide and the conjugation process was confirmed by FT-IR, SDS-PAGE, and UV spectrophotometry. Physical characterization was performed by DLS analysis and atomic force microscopy. The cytotoxicity and cellular uptake of the final construct (CGO-PEG-bLF) was inspected on lung cancer TC-1 cells by MTT assay and flow cytometry/confocal microscopy. The anticancer mechanism of the CGO-PEG-bLF was studied by cell cycle analysis, apoptosis assay, and western blot technique. Finally, the anticancer activity of CGO-PEG-bLF was assessed in an animal model of lung cancer. Size and zeta potential of CGO-PEG-bLF was obtained in the optimum range. Compared with free bLF, more cytotoxic activity, cellular uptake and more survival time was obtained for CGO-PEG-bLF. CGO-PEG-bLF significantly inhibited tumor growth in the animal model. Cell cycle arrest and apoptosis were more induced by CGO-PEG-bLF. Moreover, exposure to CGO-PEG-bLF decreased the phospho-AKT and pro-Caspase 3 levels and increased the amount of cleaved caspase 3 in the treated cells. This study revealed the potential of CGO-PEG as a promising nanocarrier for enhancing the therapeutic efficacy of anticancer agents. Taylor & Francis 2020-08-19 /pmc/articles/PMC7470100/ /pubmed/32812454 http://dx.doi.org/10.1080/10717544.2020.1809558 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Najmafshar, Azam Rostami, Mahboubeh Varshosaz, Jaleh Norouzian, Dariush Samsam Shariat, Seyed Ziyae Aldin Enhanced antitumor activity of bovine lactoferrin through immobilization onto functionalized nano graphene oxide: an in vitro/in vivo study |
title | Enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
title_full | Enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
title_fullStr | Enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
title_full_unstemmed | Enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
title_short | Enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
title_sort | enhanced antitumor activity of bovine lactoferrin through immobilization onto
functionalized nano graphene oxide: an in vitro/in vivo study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470100/ https://www.ncbi.nlm.nih.gov/pubmed/32812454 http://dx.doi.org/10.1080/10717544.2020.1809558 |
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