Phylogenomics insights into order and families of Lysobacterales

Order Lysobacterales (earlier known Xanthomonadales ) is a taxonomically complex group of a large number of gamma-proteobacteria classified in two different families, namely Lysobacteraceae and Rhodanobacteraceae . Current taxonomy is largely based on classical approaches and is devoid of whole-genome information-based analysis. In the present study, we have taken all classified and poorly described species belonging to the order Lysobacterales to perform a phylogenetic analysis based on the 16 S rRNA sequence. Moreover, to obtain robust phylogeny, we have generated whole-genome sequencing data of six type species namely Metallibacterium scheffleri , Panacagrimonas perspica , Thermomonas haemolytica , Fulvimonas soli , Pseudofulvimonas gallinarii and Rhodanobacter lindaniclasticus of the families Lysobacteraceae and Rhodanobacteraceae . Interestingly, whole-genome-based phylogenetic analysis revealed unusual positioning of the type species Pseudofulvimonas , Panacagrimonas , Metallibacterium and Aquimonas at family level. Whole-genome-based phylogeny involving 92 type strains resolved the taxonomic positioning by reshuffling the genus across families Lysobacteraceae and Rhodanobacteraceae . The present study reveals the need and scope for genome-based phylogenetic and comparative studies in order to address relationships of genera and species of order Lysobacterales .