Fine-tuning sugar content in strawberry

Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1...

Descripción completa

Detalles Bibliográficos
Autores principales: Xing, Sinian, Chen, Kunling, Zhu, Haocheng, Zhang, Rui, Zhang, Huawei, Li, Bingbing, Gao, Caixia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470447/
https://www.ncbi.nlm.nih.gov/pubmed/32883370
http://dx.doi.org/10.1186/s13059-020-02146-5
_version_ 1783578591129239552
author Xing, Sinian
Chen, Kunling
Zhu, Haocheng
Zhang, Rui
Zhang, Huawei
Li, Bingbing
Gao, Caixia
author_facet Xing, Sinian
Chen, Kunling
Zhu, Haocheng
Zhang, Rui
Zhang, Huawei
Li, Bingbing
Gao, Caixia
author_sort Xing, Sinian
collection PubMed
description Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9–83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement.
format Online
Article
Text
id pubmed-7470447
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-74704472020-09-08 Fine-tuning sugar content in strawberry Xing, Sinian Chen, Kunling Zhu, Haocheng Zhang, Rui Zhang, Huawei Li, Bingbing Gao, Caixia Genome Biol Method Fine-tuning quantitative traits for continuous subtle phenotypes is highly advantageous. We engineer the highly conserved upstream open reading frame (uORF) of FvebZIPs1.1 in strawberry (Fragaria vesca), using base editor A3A-PBE. Seven novel alleles are generated. Sugar content of the homozygous T1 mutant lines is 33.9–83.6% higher than that of the wild-type. We also recover a series of transgene-free mutants with 35 novel genotypes containing a continuum of sugar content. All the novel genotypes could be immediately fixed in subsequent generations by asexual reproduction. Genome editing coupled with asexual reproduction offers tremendous opportunities for quantitative trait improvement. BioMed Central 2020-09-03 /pmc/articles/PMC7470447/ /pubmed/32883370 http://dx.doi.org/10.1186/s13059-020-02146-5 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Method
Xing, Sinian
Chen, Kunling
Zhu, Haocheng
Zhang, Rui
Zhang, Huawei
Li, Bingbing
Gao, Caixia
Fine-tuning sugar content in strawberry
title Fine-tuning sugar content in strawberry
title_full Fine-tuning sugar content in strawberry
title_fullStr Fine-tuning sugar content in strawberry
title_full_unstemmed Fine-tuning sugar content in strawberry
title_short Fine-tuning sugar content in strawberry
title_sort fine-tuning sugar content in strawberry
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470447/
https://www.ncbi.nlm.nih.gov/pubmed/32883370
http://dx.doi.org/10.1186/s13059-020-02146-5
work_keys_str_mv AT xingsinian finetuningsugarcontentinstrawberry
AT chenkunling finetuningsugarcontentinstrawberry
AT zhuhaocheng finetuningsugarcontentinstrawberry
AT zhangrui finetuningsugarcontentinstrawberry
AT zhanghuawei finetuningsugarcontentinstrawberry
AT libingbing finetuningsugarcontentinstrawberry
AT gaocaixia finetuningsugarcontentinstrawberry

Ejemplares similares