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Parasitic Infection Surveillance in Mississippi Delta Children

Some recent studies suggest ongoing transmission of parasitic diseases in the American South; however, surveys in Mississippi children are lacking. We enrolled 166 children (median age 8 years, range 4–13 years) from the Mississippi Delta region and carried out multi-parallel real-time polymerase ch...

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Autores principales: Bradbury, Richard S., Arguello, Irene, Lane, Meredith, Cooley, Gretchen, Handali, Sukwan, Dimitrova, Silvia D., Nascimento, Fernanda S., Jameson, Sam, Hellmann, Kathryn, Tharp, Michelle, Byers, Paul, Montgomery, Susan P., Haynie, Lisa, Kirmse, Brian, Pilotte, Nils, Williams, Steven A., Hobbs, Charlotte V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470556/
https://www.ncbi.nlm.nih.gov/pubmed/32588796
http://dx.doi.org/10.4269/ajtmh.20-0026
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author Bradbury, Richard S.
Arguello, Irene
Lane, Meredith
Cooley, Gretchen
Handali, Sukwan
Dimitrova, Silvia D.
Nascimento, Fernanda S.
Jameson, Sam
Hellmann, Kathryn
Tharp, Michelle
Byers, Paul
Montgomery, Susan P.
Haynie, Lisa
Kirmse, Brian
Pilotte, Nils
Williams, Steven A.
Hobbs, Charlotte V.
author_facet Bradbury, Richard S.
Arguello, Irene
Lane, Meredith
Cooley, Gretchen
Handali, Sukwan
Dimitrova, Silvia D.
Nascimento, Fernanda S.
Jameson, Sam
Hellmann, Kathryn
Tharp, Michelle
Byers, Paul
Montgomery, Susan P.
Haynie, Lisa
Kirmse, Brian
Pilotte, Nils
Williams, Steven A.
Hobbs, Charlotte V.
author_sort Bradbury, Richard S.
collection PubMed
description Some recent studies suggest ongoing transmission of parasitic diseases in the American South; however, surveys in Mississippi children are lacking. We enrolled 166 children (median age 8 years, range 4–13 years) from the Mississippi Delta region and carried out multi-parallel real-time polymerase chain reaction (PCR) for Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis on their stool samples. Dried blood spots were obtained for multiplex serology antibody detection. Of 166 children, all reported having flushable toilets, 11% had soil exposure, and 34% had a pet dog or cat. None had prior diagnosis or treatment of parasitic disease. Multi-parallel real-time PCRs were negative on the 89 stool DNA extracts available for testing. Dried blood spot testing of all 166 children determined the seroprevalence of IgG antibodies to Toxocara spp. (3.6%), Cryptosporidium (2.4%), S. stercoralis, Fasciola hepatica, and Giardia duodenalis (all 0%). In conclusion, parasitic infections and exposure were scarce in this population. Larger studies of at-risk populations are needed.
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spelling pubmed-74705562020-09-04 Parasitic Infection Surveillance in Mississippi Delta Children Bradbury, Richard S. Arguello, Irene Lane, Meredith Cooley, Gretchen Handali, Sukwan Dimitrova, Silvia D. Nascimento, Fernanda S. Jameson, Sam Hellmann, Kathryn Tharp, Michelle Byers, Paul Montgomery, Susan P. Haynie, Lisa Kirmse, Brian Pilotte, Nils Williams, Steven A. Hobbs, Charlotte V. Am J Trop Med Hyg Articles Some recent studies suggest ongoing transmission of parasitic diseases in the American South; however, surveys in Mississippi children are lacking. We enrolled 166 children (median age 8 years, range 4–13 years) from the Mississippi Delta region and carried out multi-parallel real-time polymerase chain reaction (PCR) for Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis on their stool samples. Dried blood spots were obtained for multiplex serology antibody detection. Of 166 children, all reported having flushable toilets, 11% had soil exposure, and 34% had a pet dog or cat. None had prior diagnosis or treatment of parasitic disease. Multi-parallel real-time PCRs were negative on the 89 stool DNA extracts available for testing. Dried blood spot testing of all 166 children determined the seroprevalence of IgG antibodies to Toxocara spp. (3.6%), Cryptosporidium (2.4%), S. stercoralis, Fasciola hepatica, and Giardia duodenalis (all 0%). In conclusion, parasitic infections and exposure were scarce in this population. Larger studies of at-risk populations are needed. The American Society of Tropical Medicine and Hygiene 2020-09 2020-06-22 /pmc/articles/PMC7470556/ /pubmed/32588796 http://dx.doi.org/10.4269/ajtmh.20-0026 Text en © The American Society of Tropical Medicine and Hygiene This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Articles
Bradbury, Richard S.
Arguello, Irene
Lane, Meredith
Cooley, Gretchen
Handali, Sukwan
Dimitrova, Silvia D.
Nascimento, Fernanda S.
Jameson, Sam
Hellmann, Kathryn
Tharp, Michelle
Byers, Paul
Montgomery, Susan P.
Haynie, Lisa
Kirmse, Brian
Pilotte, Nils
Williams, Steven A.
Hobbs, Charlotte V.
Parasitic Infection Surveillance in Mississippi Delta Children
title Parasitic Infection Surveillance in Mississippi Delta Children
title_full Parasitic Infection Surveillance in Mississippi Delta Children
title_fullStr Parasitic Infection Surveillance in Mississippi Delta Children
title_full_unstemmed Parasitic Infection Surveillance in Mississippi Delta Children
title_short Parasitic Infection Surveillance in Mississippi Delta Children
title_sort parasitic infection surveillance in mississippi delta children
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470556/
https://www.ncbi.nlm.nih.gov/pubmed/32588796
http://dx.doi.org/10.4269/ajtmh.20-0026
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