Temporal Change of SARS-CoV-2 in Clinical Specimens of COVID-19 Pneumonia Patients

The quality and type of specimen collection affect the sensitivity of real-time reverse transcriptase–PCR (rRT-PCR) for the diagnosis of SARS-CoV-2. In this report, the course over time of rRT-PCR for SARS-CoV-2 in 26 clinical specimens collected from the upper (nasopharyngeal and throat swabs) and...

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Detalles Bibliográficos
Autores principales: Luvira, Viravarn, Jittmittraphap, Akanitt, Muangnoicharoen, Sant, Chantawat, Nantarat, Janwitthayanan, Weena, Leaungwutiwong, Pornsawan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society of Tropical Medicine and Hygiene 2020
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470564/
https://www.ncbi.nlm.nih.gov/pubmed/32662396
http://dx.doi.org/10.4269/ajtmh.20-0551
Descripción
Sumario:The quality and type of specimen collection affect the sensitivity of real-time reverse transcriptase–PCR (rRT-PCR) for the diagnosis of SARS-CoV-2. In this report, the course over time of rRT-PCR for SARS-CoV-2 in 26 clinical specimens collected from the upper (nasopharyngeal and throat swabs) and lower (sputum) respiratory tracts of COVID-19 cases with pneumonia was investigated along with the clinical course. The preliminary results revealed that higher SARS-CoV-2 RNA concentration and longer time for detection make self-collected sputum a preferable specimen for the diagnosis and follow-up of COVID-19 pneumonia. Self-collection of sputum can minimize the risk of unnecessary exposure to healthcare workers, preserve the shortage of personal protective equipment, and limit viral transmission to the environment.

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