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Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape

BACKGROUND: Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding...

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Autores principales: Wang, Huiling, Yan, Ailing, Sun, Lei, Zhang, Guojun, Wang, Xiaoyue, Ren, Jiancheng, Xu, Haiying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470616/
https://www.ncbi.nlm.nih.gov/pubmed/32883214
http://dx.doi.org/10.1186/s12870-020-02630-x
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author Wang, Huiling
Yan, Ailing
Sun, Lei
Zhang, Guojun
Wang, Xiaoyue
Ren, Jiancheng
Xu, Haiying
author_facet Wang, Huiling
Yan, Ailing
Sun, Lei
Zhang, Guojun
Wang, Xiaoyue
Ren, Jiancheng
Xu, Haiying
author_sort Wang, Huiling
collection PubMed
description BACKGROUND: Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding in grapevine. Mapping and isolation of quantitative trait locus (QTLs) based on the construction of genetic linkage map is a powerful approach to decipher the genetic determinants of complex quantitative traits. RESULTS: In the present work, a final integrated map consisting of 3411 SLAF markers on 19 linkage groups (LGs) with an average distance of 0.98 cM between adjacent markers was generated using the specific length amplified fragment sequencing (SLAF-seq) technique. A total of 9 significant stable QTLs for Muscat flavor, berry firmness and berry shape were identified on two linkage groups among the hybrids analyzed over three consecutive years from 2016 to 2018. Notably, new stable QTLs for berry firmness and berry shape were found on LG 8 respectively for the first time. Based on biological function and expression profiles of candidate genes in the major QTL regions, 3 genes (VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350) related to berry firmness and 3 genes (VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200) linked to berry shape were highlighted. Overexpression of VIT_08s0032g01110 in transgenic Arabidopsis plants caused the change of pod shape. CONCLUSIONS: A new high-density genetic map with total 3411 markers was constructed with SLAF-seq technique, and thus enabled the detection of narrow interval QTLs for relevant traits in grapevine. VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350 were found to be related to berry firmness, while VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200 were linked to berry shape.
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spelling pubmed-74706162020-09-08 Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape Wang, Huiling Yan, Ailing Sun, Lei Zhang, Guojun Wang, Xiaoyue Ren, Jiancheng Xu, Haiying BMC Plant Biol Research Article BACKGROUND: Aroma, berry firmness and berry shape are three main quality traits in table grape production, and also the important target traits in grapevine breeding. However, the information about their genetic mechanisms is limited, which results in low accuracy and efficiency of quality breeding in grapevine. Mapping and isolation of quantitative trait locus (QTLs) based on the construction of genetic linkage map is a powerful approach to decipher the genetic determinants of complex quantitative traits. RESULTS: In the present work, a final integrated map consisting of 3411 SLAF markers on 19 linkage groups (LGs) with an average distance of 0.98 cM between adjacent markers was generated using the specific length amplified fragment sequencing (SLAF-seq) technique. A total of 9 significant stable QTLs for Muscat flavor, berry firmness and berry shape were identified on two linkage groups among the hybrids analyzed over three consecutive years from 2016 to 2018. Notably, new stable QTLs for berry firmness and berry shape were found on LG 8 respectively for the first time. Based on biological function and expression profiles of candidate genes in the major QTL regions, 3 genes (VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350) related to berry firmness and 3 genes (VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200) linked to berry shape were highlighted. Overexpression of VIT_08s0032g01110 in transgenic Arabidopsis plants caused the change of pod shape. CONCLUSIONS: A new high-density genetic map with total 3411 markers was constructed with SLAF-seq technique, and thus enabled the detection of narrow interval QTLs for relevant traits in grapevine. VIT_08s0007g00440, VIT_08s0040g02740 and VIT_08s0040g02350 were found to be related to berry firmness, while VIT_08s0032g01110, VIT_08s0032g01150 and VIT_08s0105g00200 were linked to berry shape. BioMed Central 2020-09-03 /pmc/articles/PMC7470616/ /pubmed/32883214 http://dx.doi.org/10.1186/s12870-020-02630-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wang, Huiling
Yan, Ailing
Sun, Lei
Zhang, Guojun
Wang, Xiaoyue
Ren, Jiancheng
Xu, Haiying
Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_full Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_fullStr Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_full_unstemmed Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_short Novel stable QTLs identification for berry quality traits based on high-density genetic linkage map construction in table grape
title_sort novel stable qtls identification for berry quality traits based on high-density genetic linkage map construction in table grape
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470616/
https://www.ncbi.nlm.nih.gov/pubmed/32883214
http://dx.doi.org/10.1186/s12870-020-02630-x
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