Application of different types of CRISPR/Cas-based systems in bacteria

As important genome editing tools, CRISPR/Cas systems, especially those based on type II Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria. However, the intrinsic toxicity of Cas9 and Cas12a-mediated CRISPR/Cas tools can lead to cell death in some strains, whic...

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Detalles Bibliográficos
Autores principales: Liu, Zhenquan, Dong, Huina, Cui, Yali, Cong, Lina, Zhang, Dawei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470686/
https://www.ncbi.nlm.nih.gov/pubmed/32883277
http://dx.doi.org/10.1186/s12934-020-01431-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470686/
https://www.ncbi.nlm.nih.gov/pubmed/32883277
http://dx.doi.org/10.1186/s12934-020-01431-z

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