Cargando…

circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis

BACKGROUND: Growing evidence has revealed the involvement of circular RNAs (circRNAs) in numerous carcinogenesis. However, the role of circRNAs in the cancer biology of colorectal cancer (CRC) remains vague. METHODS: Quantitative RT-PCR was used to detect the expression level of circRAE1 in CRC tiss...

Descripción completa

Detalles Bibliográficos
Autores principales: Du, Jiabin, Xu, Jianhua, Chen, Junxing, Liu, Weinan, Wang, Pengcheng, Ye, Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470687/
https://www.ncbi.nlm.nih.gov/pubmed/32908453
http://dx.doi.org/10.1186/s12935-020-01519-x
_version_ 1783578626433744896
author Du, Jiabin
Xu, Jianhua
Chen, Junxing
Liu, Weinan
Wang, Pengcheng
Ye, Kai
author_facet Du, Jiabin
Xu, Jianhua
Chen, Junxing
Liu, Weinan
Wang, Pengcheng
Ye, Kai
author_sort Du, Jiabin
collection PubMed
description BACKGROUND: Growing evidence has revealed the involvement of circular RNAs (circRNAs) in numerous carcinogenesis. However, the role of circRNAs in the cancer biology of colorectal cancer (CRC) remains vague. METHODS: Quantitative RT-PCR was used to detect the expression level of circRAE1 in CRC tissues and CRC cell lines. Cell proliferation, migration, and invasion were detected using CCK8 assay, Colony formation assay, wound-healing and Transwell assays. The interaction between circRAE1 and miR-338-3p and TRYO3 was confirmed using dual-luciferase reporter assays. RESULTS: We uncovered a novel circRNA Hsa_circ_0060967 (also known as circRAE1) that was remarkably increased in CRC tissues. The high circRAE1 level was positively associated with advanced tumor stage, lymph node metastasis, and tumor size. The loss-of-function assay showed that circRAE1 accelerated cell proliferation, migration, and invasion. Besides, miR-338-3p was lowly expressed in the CRC tissues and CRC cell lines. The dual-luciferase reporter assays showed that circRAE1 could sponge miR-338-3p, which targeted TRYO3 in CRC cells. Furthermore, the overexpression of circRAE1 could rescue the impaired migration and invasion triggered by miR-338-3p mimics or si-TYRO3 in CRC cells and vice versa. CONCLUSION: We identified the network of circRAE1, miR-338-3p, and TYRO3 in CRC cells and determined that the increase in circRAE1 could serve as an oncogene by sponging miR-338-3p, which resulted in an upregulated TYRO3 expression. The finding suggests that circRAE1 is a potential therapeutic target and diagnostic marker for CRC treatment.
format Online
Article
Text
id pubmed-7470687
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-74706872020-09-08 circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis Du, Jiabin Xu, Jianhua Chen, Junxing Liu, Weinan Wang, Pengcheng Ye, Kai Cancer Cell Int Primary Research BACKGROUND: Growing evidence has revealed the involvement of circular RNAs (circRNAs) in numerous carcinogenesis. However, the role of circRNAs in the cancer biology of colorectal cancer (CRC) remains vague. METHODS: Quantitative RT-PCR was used to detect the expression level of circRAE1 in CRC tissues and CRC cell lines. Cell proliferation, migration, and invasion were detected using CCK8 assay, Colony formation assay, wound-healing and Transwell assays. The interaction between circRAE1 and miR-338-3p and TRYO3 was confirmed using dual-luciferase reporter assays. RESULTS: We uncovered a novel circRNA Hsa_circ_0060967 (also known as circRAE1) that was remarkably increased in CRC tissues. The high circRAE1 level was positively associated with advanced tumor stage, lymph node metastasis, and tumor size. The loss-of-function assay showed that circRAE1 accelerated cell proliferation, migration, and invasion. Besides, miR-338-3p was lowly expressed in the CRC tissues and CRC cell lines. The dual-luciferase reporter assays showed that circRAE1 could sponge miR-338-3p, which targeted TRYO3 in CRC cells. Furthermore, the overexpression of circRAE1 could rescue the impaired migration and invasion triggered by miR-338-3p mimics or si-TYRO3 in CRC cells and vice versa. CONCLUSION: We identified the network of circRAE1, miR-338-3p, and TYRO3 in CRC cells and determined that the increase in circRAE1 could serve as an oncogene by sponging miR-338-3p, which resulted in an upregulated TYRO3 expression. The finding suggests that circRAE1 is a potential therapeutic target and diagnostic marker for CRC treatment. BioMed Central 2020-09-03 /pmc/articles/PMC7470687/ /pubmed/32908453 http://dx.doi.org/10.1186/s12935-020-01519-x Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Du, Jiabin
Xu, Jianhua
Chen, Junxing
Liu, Weinan
Wang, Pengcheng
Ye, Kai
circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title_full circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title_fullStr circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title_full_unstemmed circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title_short circRAE1 promotes colorectal cancer cell migration and invasion by modulating miR-338-3p/TYRO3 axis
title_sort circrae1 promotes colorectal cancer cell migration and invasion by modulating mir-338-3p/tyro3 axis
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470687/
https://www.ncbi.nlm.nih.gov/pubmed/32908453
http://dx.doi.org/10.1186/s12935-020-01519-x
work_keys_str_mv AT dujiabin circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis
AT xujianhua circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis
AT chenjunxing circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis
AT liuweinan circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis
AT wangpengcheng circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis
AT yekai circrae1promotescolorectalcancercellmigrationandinvasionbymodulatingmir3383ptyro3axis