Cargando…
Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium
Controlled ovarian hyperstimulation (COH) is routinary used in assisted reproductive technologies (ARTs) to increase the yields of mature oocytes. The possibility that patients with a history of failures or poor-responders may develop side-effects following these treatments is still debated. Epidemi...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Society for Reproduction and Development
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470905/ https://www.ncbi.nlm.nih.gov/pubmed/32350229 http://dx.doi.org/10.1262/jrd.2019-147 |
_version_ | 1783578670982496256 |
---|---|
author | ANTONOULI, Sevastiani PALMERINI, Maria Grazia BIANCHI, Serena ROSSI, Gianna CECCONI, Sandra BELLI, Manuel BERNARDI, Sara KHALILI, Mohammad Ali FAMILIARI, Giuseppe NOTTOLA, Stefania Annarita MACCHIARELLI, Guido |
author_facet | ANTONOULI, Sevastiani PALMERINI, Maria Grazia BIANCHI, Serena ROSSI, Gianna CECCONI, Sandra BELLI, Manuel BERNARDI, Sara KHALILI, Mohammad Ali FAMILIARI, Giuseppe NOTTOLA, Stefania Annarita MACCHIARELLI, Guido |
author_sort | ANTONOULI, Sevastiani |
collection | PubMed |
description | Controlled ovarian hyperstimulation (COH) is routinary used in assisted reproductive technologies (ARTs) to increase the yields of mature oocytes. The possibility that patients with a history of failures or poor-responders may develop side-effects following these treatments is still debated. Epidemiological studies reported controversial results about pregnancy outcome and the risk of developing gynecological cancers. By using a mouse model, here we compared the ultrastructural features of fallopian tubes (FTs) obtained from mice undergoing or not (control, CTR) four (4R) and eight (8R) rounds of gonadotropin stimulation. Although the morphological characteristics of oviductal layers seemed unaffected by repeated treatments, dose-response ultrastructural alterations in the ampulla appeared in the 4R group and even more in the 8R group. The targets were oviductal ciliated (CCs) and non-ciliated (NCCs) cells, which showed damaged mitochondria and glycogen accumulations in the cytoplasm. The drastic reduction of CCs, evident after 4R, was supported by the absence of cilia. After 8R, glycogen granules were significantly reduced and massive degeneration of mitochondria, which appeared swollen and/or vacuolated, occurred in NCCs. Moreover, disintegrated mitochondria were found at the periphery of mitophagic vacuoles with evident signs of cristolysis. The morphometric analysis evidenced a significant increase in the density and frequency of damaged mitochondria after 4R and 8R. The absence of cilia, necessary to sustain oviductal transport of oocytes, spermatozoa and embryos, may originate from either mitochondrial dysfunction or glycogen consumption. These results suggest that repeated COH treatments could induce alterations impairing fertilization and embryo transport toward the uterus. |
format | Online Article Text |
id | pubmed-7470905 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Society for Reproduction and Development |
record_format | MEDLINE/PubMed |
spelling | pubmed-74709052020-09-09 Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium ANTONOULI, Sevastiani PALMERINI, Maria Grazia BIANCHI, Serena ROSSI, Gianna CECCONI, Sandra BELLI, Manuel BERNARDI, Sara KHALILI, Mohammad Ali FAMILIARI, Giuseppe NOTTOLA, Stefania Annarita MACCHIARELLI, Guido J Reprod Dev Original Article Controlled ovarian hyperstimulation (COH) is routinary used in assisted reproductive technologies (ARTs) to increase the yields of mature oocytes. The possibility that patients with a history of failures or poor-responders may develop side-effects following these treatments is still debated. Epidemiological studies reported controversial results about pregnancy outcome and the risk of developing gynecological cancers. By using a mouse model, here we compared the ultrastructural features of fallopian tubes (FTs) obtained from mice undergoing or not (control, CTR) four (4R) and eight (8R) rounds of gonadotropin stimulation. Although the morphological characteristics of oviductal layers seemed unaffected by repeated treatments, dose-response ultrastructural alterations in the ampulla appeared in the 4R group and even more in the 8R group. The targets were oviductal ciliated (CCs) and non-ciliated (NCCs) cells, which showed damaged mitochondria and glycogen accumulations in the cytoplasm. The drastic reduction of CCs, evident after 4R, was supported by the absence of cilia. After 8R, glycogen granules were significantly reduced and massive degeneration of mitochondria, which appeared swollen and/or vacuolated, occurred in NCCs. Moreover, disintegrated mitochondria were found at the periphery of mitophagic vacuoles with evident signs of cristolysis. The morphometric analysis evidenced a significant increase in the density and frequency of damaged mitochondria after 4R and 8R. The absence of cilia, necessary to sustain oviductal transport of oocytes, spermatozoa and embryos, may originate from either mitochondrial dysfunction or glycogen consumption. These results suggest that repeated COH treatments could induce alterations impairing fertilization and embryo transport toward the uterus. The Society for Reproduction and Development 2020-04-28 2020-08 /pmc/articles/PMC7470905/ /pubmed/32350229 http://dx.doi.org/10.1262/jrd.2019-147 Text en ©2020 Society for Reproduction and Development This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Original Article ANTONOULI, Sevastiani PALMERINI, Maria Grazia BIANCHI, Serena ROSSI, Gianna CECCONI, Sandra BELLI, Manuel BERNARDI, Sara KHALILI, Mohammad Ali FAMILIARI, Giuseppe NOTTOLA, Stefania Annarita MACCHIARELLI, Guido Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title | Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title_full | Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title_fullStr | Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title_full_unstemmed | Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title_short | Repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
title_sort | repeated hyperstimulation affects the ultrastructure of mouse fallopian tube epithelium |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7470905/ https://www.ncbi.nlm.nih.gov/pubmed/32350229 http://dx.doi.org/10.1262/jrd.2019-147 |
work_keys_str_mv | AT antonoulisevastiani repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT palmerinimariagrazia repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT bianchiserena repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT rossigianna repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT cecconisandra repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT bellimanuel repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT bernardisara repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT khalilimohammadali repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT familiarigiuseppe repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT nottolastefaniaannarita repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium AT macchiarelliguido repeatedhyperstimulationaffectstheultrastructureofmousefallopiantubeepithelium |