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A Simplified Quantitative Real-Time PCR Assay for Monitoring SARS-CoV-2 Growth in Cell Culture
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected millions within just a few months, causing severe respiratory disease and mortality. Assays to monitor SARS-CoV-2 growth in vitro depend on time-consuming and costly RNA extraction steps, hampering progress in basic research a...
Autores principales: | Shema Mugisha, Christian, Vuong, Hung R., Puray-Chavez, Maritza, Bailey, Adam L., Fox, Julie M., Chen, Rita E., Wessel, Alex W., Scott, Jason M., Harastani, Houda H., Boon, Adrianus C. M., Shin, Haina, Kutluay, Sebla B. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471006/ https://www.ncbi.nlm.nih.gov/pubmed/32878932 http://dx.doi.org/10.1128/mSphere.00658-20 |
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