Insufficiently complex unique-molecular identifiers (UMIs) distort small RNA sequencing

The attachment of unique molecular identifiers (UMIs) to RNA molecules prior to PCR amplification and sequencing, makes it possible to amplify libraries to a level that is sufficient to identify rare molecules, whilst simultaneously eliminating PCR bias through the identification of duplicated reads...

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Detalles Bibliográficos
Autores principales: Saunders, Klay, Bert, Andrew G., Dredge, B. Kate, Toubia, John, Gregory, Philip A., Pillman, Katherine A., Goodall, Gregory J., Bracken, Cameron P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471316/
https://www.ncbi.nlm.nih.gov/pubmed/32884024
http://dx.doi.org/10.1038/s41598-020-71323-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471316/
https://www.ncbi.nlm.nih.gov/pubmed/32884024
http://dx.doi.org/10.1038/s41598-020-71323-0

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