Cargando…
Insufficiently complex unique-molecular identifiers (UMIs) distort small RNA sequencing
The attachment of unique molecular identifiers (UMIs) to RNA molecules prior to PCR amplification and sequencing, makes it possible to amplify libraries to a level that is sufficient to identify rare molecules, whilst simultaneously eliminating PCR bias through the identification of duplicated reads...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2020
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471316/ https://www.ncbi.nlm.nih.gov/pubmed/32884024 http://dx.doi.org/10.1038/s41598-020-71323-0 |