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Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure

In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons a...

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Autores principales: Törnquist, Elin, Gentile, Luigi, Prévost, Sylvain, Diaz, Ana, Olsson, Ulf, Isaksson, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471706/
https://www.ncbi.nlm.nih.gov/pubmed/32883964
http://dx.doi.org/10.1038/s41598-020-71190-9
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author Törnquist, Elin
Gentile, Luigi
Prévost, Sylvain
Diaz, Ana
Olsson, Ulf
Isaksson, Hanna
author_facet Törnquist, Elin
Gentile, Luigi
Prévost, Sylvain
Diaz, Ana
Olsson, Ulf
Isaksson, Hanna
author_sort Törnquist, Elin
collection PubMed
description In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons and X-rays. For X-rays, it is proportional to the electron density while for neutrons it varies irregularly with the atomic number. Hence, combining the two techniques on the same specimens allows for a more detailed interpretation of the scattering patterns as compared to a single-contrast experiment. The current study was performed on bovine, porcine and ovine specimens, obtained in two perpendicular directions with respect to the main axis of the bone (longitudinal and radial) in order to maximise the understanding of the nanostructural organisation. The specimens were also imaged with high resolution micro-computed tomography (micro-CT), yielding tissue mineral density and microstructural orientation as reference. We show that the SANS and SAXS patterns from the same specimen are effectively identical, suggesting that these bone specimens can be approximated as a two-component composite material. Hence, the observed small-angle scattering results mainly from the mineral-collagen contrast, apart from minor features associated with the internal collagen structure.
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spelling pubmed-74717062020-09-04 Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure Törnquist, Elin Gentile, Luigi Prévost, Sylvain Diaz, Ana Olsson, Ulf Isaksson, Hanna Sci Rep Article In this study, we present a combined small-angle neutron and X-ray scattering (SANS and SAXS) study of the nanoscale structure of cortical bone specimens from three different species. The variation of the scattering cross section of elements across the periodic table is very different for neutrons and X-rays. For X-rays, it is proportional to the electron density while for neutrons it varies irregularly with the atomic number. Hence, combining the two techniques on the same specimens allows for a more detailed interpretation of the scattering patterns as compared to a single-contrast experiment. The current study was performed on bovine, porcine and ovine specimens, obtained in two perpendicular directions with respect to the main axis of the bone (longitudinal and radial) in order to maximise the understanding of the nanostructural organisation. The specimens were also imaged with high resolution micro-computed tomography (micro-CT), yielding tissue mineral density and microstructural orientation as reference. We show that the SANS and SAXS patterns from the same specimen are effectively identical, suggesting that these bone specimens can be approximated as a two-component composite material. Hence, the observed small-angle scattering results mainly from the mineral-collagen contrast, apart from minor features associated with the internal collagen structure. Nature Publishing Group UK 2020-09-03 /pmc/articles/PMC7471706/ /pubmed/32883964 http://dx.doi.org/10.1038/s41598-020-71190-9 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Törnquist, Elin
Gentile, Luigi
Prévost, Sylvain
Diaz, Ana
Olsson, Ulf
Isaksson, Hanna
Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title_full Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title_fullStr Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title_full_unstemmed Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title_short Comparison of small-angle neutron and X-ray scattering for studying cortical bone nanostructure
title_sort comparison of small-angle neutron and x-ray scattering for studying cortical bone nanostructure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7471706/
https://www.ncbi.nlm.nih.gov/pubmed/32883964
http://dx.doi.org/10.1038/s41598-020-71190-9
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